稀土学报(英文版)
稀土學報(英文版)
희토학보(영문판)
JOURNAL OF RARE EARTHS
2004年
6期
891-895
,共5页
张金超%张天蓝%许善锦%王夔%于世凤%杨梦苏
張金超%張天藍%許善錦%王夔%于世鳳%楊夢囌
장금초%장천람%허선금%왕기%우세봉%양몽소
lanthanum%osteoclast-like cells%1,25-dihydroxyvitamin D3%bone marrow cells%bone resorption%rare earths
The effect of La3+ on formation of osteoclast-like cells in rabbit bone marrow cells induced by 1,25-dihydroxyvitamin D3 and their bone-resorbing activity was evaluated by counting the number of tartrate resistant-acid phosphatase-positive [TRAP(+)] multi-nucleated cells and measuring the number and surface area of bone resorption pits with photomicrography and image analysis. The formation and morphological characteristics of osteoclast-like cells and bone resorption pits were observed under a phase contrast inverted microscope. La3+ promotes the formation of osteoclast-like cells at the concentration of 1.00×10-8mol·L-1 compared with the control group(P<0.01), whereas no significant change in cell number is observed at higher concentrations(1.00×10-5, 1.00×10-6 and 1.00×10-7 mol·L-1)(P>0.05). La3+ at the concentration of 1.00×10-8mol·L-1 also increases the number and surface area of the resorption pits(P<0.01), but inhibits the bone-resorbing activity dose-dependently(P<0.01)at higher concentrations(1.00×10-5, 1.00×10-6 and 1.00×10-7 mol·L-1). These findings suggest that La3+ may promote or inhibit the formation and bone-resorbing activity of osteoclast-like cells depending on its concentration.