CAJ | 학술논문

利用Design Expert软件、采用Plackett-Burman(PB)实验设计和响应面分析法,对白腐菌Y10产漆酶的培养基进行了优化研究,结果表明:大量元素、氨三乙酸(NTA)和藜芦醇(VA)是影响漆酶酶活的3个主要因素;在此基础上,用最陡爬坡路径逼近最大响应区域,并利用Box-Behnken实验设计及响应面分析法确定了主要影响因素的最佳条件,即白腐菌Y10产漆酶最佳培养基组分为葡萄糖10.00 g,L、酒石酸铵0.50 g/L、大量元素296.50 ml/L、微量元素100.00 ml/L、NTA1.40g/L、VA 5.00 mm01/L、吐温-80加入量为0.10%,在该优化培养基中漆酶酶活达5 282.56 U/L,约为优化前的23倍,其实验值与理论值基本相符.
이용Design Expert연건、채용Plackett-Burman(PB)실험설계화향응면분석법,대백부균Y10산칠매적배양기진행료우화연구,결과표명:대량원소、안삼을산(NTA)화려호순(VA)시영향칠매매활적3개주요인소;재차기출상,용최두파파로경핍근최대향응구역,병이용Box-Behnken실험설계급향응면분석법학정료주요영향인소적최가조건,즉백부균Y10산칠매최가배양기조분위포도당10.00 g,L、주석산안0.50 g/L、대량원소296.50 ml/L、미량원소100.00 ml/L、NTA1.40g/L、VA 5.00 mm01/L、토온-80가입량위0.10%,재해우화배양기중칠매매활체5 282.56 U/L,약위우화전적23배,기실험치여이론치기본상부.
Plackett-Burman (PB)design and response surface methodology were applied to optimize the culture medium of laccase by white-rot fungus Y10. The results indicate that macro elements, NTA, VA, as three factors, were found to be significant to the yield and activity of laccase. The pass of steepest ascent was undertaken to approach the optimal region of the three significant factors. Box-Behnken design and response surface analysis were adopted to further investigate the mutual interaction between the variables and identify optimal values that bring maximum laccase activity. Under the optimal conditions, the activity of laccase increased nearly 23 times to 5282.56U/L and the experimental values agreed with the predicted values.