CAJ | 학술논문

目的:研究黄酮类化合物木犀草素(Luteolin)对体外抗肿瘤细胞增殖,以及其与抗肿瘤药联用的增敏作用.方法:选用5 μmol/L或10 μmol/L木犀草素与不同浓度抗肿瘤药联合作用肿瘤细胞24 h后,用MTS法检测其体外抗增殖作用.结果:5 μmol/L木犀草素对人非小细胞肺癌细胞(A549)、人子宫颈癌细胞(Hela)、人乳腺癌细胞(MCF-7)、人胃腺癌细胞(AGS)、人胃癌细胞(MGC-803)的抗增殖作用均<20%,对人结肠癌细胞(Caco2)和人肝癌细胞(HepG2)的抗增殖作用约20%.Bexarotene单一用药时对Hela细胞抑制率达50%(IC_(50))的浓度约为2 μmol/L,与5 μmol/L木犀草素联用后IC_(50)约0.2 μmol/L,5 μmol/L木犀草素与0.1 μmol/L Bexarotene联用对Hela细胞的抗增殖作用达44%,Bexarotene与木犀草素联用在MGC-803、HepG2、A549细胞中增敏较小,在Caco2和MCF-7细胞中无增敏作用;顺铂单一用药时对Hela细胞的IC_(50 )>30 μmol/L,与5 μmol/L木犀草素联用后IC_(50)约3 μmol/L,联用后在MGC-803、HepG2和A549中增敏较小;博来霉素单一用药时对Hela细胞的IC_(50)>100 μmol/L,对A549细胞的IC_(50)约为100 μmol/L,与5 μmol/L木犀草素联用后Hela细胞的IC_(50)约为1 μmol/L,与10 μmol/L木犀草素联用后A549细胞的IC_(50)约为10 μmol/L.木犀草素与格列卫联用在MGC-803、HepG2、A549和AGS中增敏较小.结论:木犀草素在低于10 μmol/L浓度时,对肿瘤细胞体外抗增殖作用较小.低浓度(5 μmol/L~10 μmol/L)的木犀草素在不同的肿瘤细胞中对抗肿瘤药的增敏作用强度不同,在Hela细胞中增敏作用最显著.
목적:연구황동류화합물목서초소(Luteolin)대체외항종류세포증식,이급기여항종류약련용적증민작용.방법:선용5 μmol/L혹10 μmol/L목서초소여불동농도항종류약연합작용종류세포24 h후,용MTS법검측기체외항증식작용.결과:5 μmol/L목서초소대인비소세포폐암세포(A549)、인자궁경암세포(Hela)、인유선암세포(MCF-7)、인위선암세포(AGS)、인위암세포(MGC-803)적항증식작용균<20%,대인결장암세포(Caco2)화인간암세포(HepG2)적항증식작용약20%.Bexarotene단일용약시대Hela세포억제솔체50%(IC_(50))적농도약위2 μmol/L,여5 μmol/L목서초소련용후IC_(50)약0.2 μmol/L,5 μmol/L목서초소여0.1 μmol/L Bexarotene련용대Hela세포적항증식작용체44%,Bexarotene여목서초소련용재MGC-803、HepG2、A549세포중증민교소,재Caco2화MCF-7세포중무증민작용;순박단일용약시대Hela세포적IC_(50 )>30 μmol/L,여5 μmol/L목서초소련용후IC_(50)약3 μmol/L,련용후재MGC-803、HepG2화A549중증민교소;박래매소단일용약시대Hela세포적IC_(50)>100 μmol/L,대A549세포적IC_(50)약위100 μmol/L,여5 μmol/L목서초소련용후Hela세포적IC_(50)약위1 μmol/L,여10 μmol/L목서초소련용후A549세포적IC_(50)약위10 μmol/L.목서초소여격렬위련용재MGC-803、HepG2、A549화AGS중증민교소.결론:목서초소재저우10 μmol/L농도시,대종류세포체외항증식작용교소.저농도(5 μmol/L~10 μmol/L)적목서초소재불동적종류세포중대항종류약적증민작용강도불동,재Hela세포중증민작용최현저.
Objective: To investigate the effect of Luteolin alone or combination with chemotherapentic drugs on the cytoxicity of cancer cells.Methods: Cultured A549,Hela,MCF-7,AGS,MGC-803,Caco2 and HepG2 cells were treated with Luteolin or the combination of Luteolin with other chemotherapeutic agents (Bexarotene,Cisplatin and Bleomycin).Cell viability was measured by MTS assay and IC_(50) was calculated.Results: The IC_(50) of Bexarotene to Hela cells was 2 μmol/L,but with the combination of 5 μmol/L of Luteolin that reduced to 0.2 μmol/L.However,the combination of Bexarotene and Luteolin did not show significant benefit in MGC-803,HepG2 cells,Caco2 and MCF-7 cells.The IC_(50) of Cisplatin to Hela cells was over 30 μmol/L,but it decreased to 3 μmol/L in the presence of 5 μmol/L Luteolin; Luteolin also sensitized Cisplatin in MGC-803,HepG2 and A549 cells studied.The IC_(50) of Bleomycin to Hela cells was over 100 μmol/L,but it was about 1 μmol/L in the presence of 5 μmol/L Luteolin.A549 cells were resistant to Bleomycin with an IC_(50) of 100 μmol/L,10 μmol/L Luteolin greatly enhanced the cytotoxicity of Bleomycin to the cells with the IC_(50) of about 10 μmol/L.The inhibitions of MGC-803,HepG2,A549 and AGS cells didn't change by combination of Luteolin.Conclusion: Low concentration of Luteolin has little toxic effect on the cancer cell lines tested in the study,but it can sensitize chemotherapeutic drugs in various cancer cell lines.