浙江大学学报（农业与生命科学版）
浙江大學學報（農業與生命科學版）
절강대학학보（농업여생명과학판）
JOURNAL OF ZHEJIANG UNIVERSITY(AGRICULTURE & LIFE SCIENCES)
2010年
1期
56-61
,共6页
韩立荣%王永宏%何军%安凤秋%张兴
韓立榮%王永宏%何軍%安鳳鞦%張興
한립영%왕영굉%하군%안봉추%장흥
枝状枝孢菌%纤维素酶%响应面法%液体发酵
枝狀枝孢菌%纖維素酶%響應麵法%液體髮酵
지상지포균%섬유소매%향응면법%액체발효
Cladosporium cladosporioides%cellulase%response surface methodology%liquid fermentation
采用响应面法对枝状枝孢菌(Cladosporium cladosporioides)产葡聚糖内切酶(CMCase)的液体发酵培养基进行优化.用单因素试验确定发酵培养基的最佳碳源、氮源和无机盐;通过中心组合试验和响应面法优化以上因素,得到的优化发酵培养基为:麦秸23.18 g·L～(-1)、麦麸30.00 g·L～(-1)、酵母膏13.62 g·L～(-1)、KH_2PO_4 4.60 g·L～(-1)、NaCl 4.60 g·L～(-1)、MgSO_4·7H_2O 0.46 g·L～(-1).在此条件下,该菌发酵液中CMCase活力达2.80 U·mL～(-1),较优化前提高了2.18倍.
採用響應麵法對枝狀枝孢菌(Cladosporium cladosporioides)產葡聚糖內切酶(CMCase)的液體髮酵培養基進行優化.用單因素試驗確定髮酵培養基的最佳碳源、氮源和無機鹽;通過中心組閤試驗和響應麵法優化以上因素,得到的優化髮酵培養基為:麥秸23.18 g·L～(-1)、麥麩30.00 g·L～(-1)、酵母膏13.62 g·L～(-1)、KH_2PO_4 4.60 g·L～(-1)、NaCl 4.60 g·L～(-1)、MgSO_4·7H_2O 0.46 g·L～(-1).在此條件下,該菌髮酵液中CMCase活力達2.80 U·mL～(-1),較優化前提高瞭2.18倍.
채용향응면법대지상지포균(Cladosporium cladosporioides)산포취당내절매(CMCase)적액체발효배양기진행우화.용단인소시험학정발효배양기적최가탄원、담원화무궤염;통과중심조합시험화향응면법우화이상인소,득도적우화발효배양기위:맥갈23.18 g·L～(-1)、맥부30.00 g·L～(-1)、효모고13.62 g·L～(-1)、KH_2PO_4 4.60 g·L～(-1)、NaCl 4.60 g·L～(-1)、MgSO_4·7H_2O 0.46 g·L～(-1).재차조건하,해균발효액중CMCase활력체2.80 U·mL～(-1),교우화전제고료2.18배.
The medium of liquid fermentation for producing CMCase (carboxymethyl cellulase) by Cladosporium cladosporioides strain F4-1 was optimized using response surface methodology (RSM). The optimum carbon, nitrogen and inorganic salts were obtained with one-factor-at-a-time. A central composite design and RSM were applied to determine the optimal concentration of each component above. The results show that the optimal medium was as follows:wheat straw 23.18 g·L～(-1),wheat bran 30.00 g·L～(-1), yeast extract 13.62 g·L～(-1),KH_2PO_4 4.60 g·L～(-1),NaCl 4.60 g·L～(-1),MgSO_4·7H_2O 0.46 g·L～(-1) . Under this condition, the CMCase activity reached 2.80 U·mL～(-1), which increased 2.18 times than before optimization.
