畜牧兽医学报
畜牧獸醫學報
축목수의학보
2010年
2期
162-166
,共5页
王存波%常国斌%徐琪%王克华%雷黎丽%周琼%廖菁%陈蓉%胡国顺%龚琳琳%陈国宏
王存波%常國斌%徐琪%王剋華%雷黎麗%週瓊%廖菁%陳蓉%鬍國順%龔琳琳%陳國宏
왕존파%상국빈%서기%왕극화%뢰려려%주경%료정%진용%호국순%공림림%진국굉
白细胞介素-1%白细胞介素-1β基因%单核苷酸多态性%鸡
白細胞介素-1%白細胞介素-1β基因%單覈苷痠多態性%鷄
백세포개소-1%백세포개소-1β기인%단핵감산다태성%계
interleukin-1%IL-1β gene%SNP%chicken
本研究旨存通过检测鸡白细胞介素-1β(interleukin-1β,IL-1β)基因部分区域单核苷酸多态位点,以期查找与血清中IL-1含量相关的遗传标记.根据红色原鸡IL-1β基因序列设计2对引物,利用PCR及单链构象多态性(Single-Strand Conformation Polymorphism,SSCP)方法对如皋鸡、文昌鸡和安卡鸡进行单核苷酸多态性(Single nucleotide polymorphism,SNP)分析,并利用试剂盒ELISA法对56日龄血清IL-1含量进行检测.结果表明血清中IL-1含量安卡鸡极显著高于如皋鸡(P<0.01)和文昌鸡(P<0.001).IL-1β基因476位点发生G→A突变、482位点发生T→C突变、1 215位点发生C→T突变、1 507位点发生T→C突变.对多态位点和IL-1含量作关联分析,只发现1 215位点与IL-1含量有关,TT基因型个体IL-1含量显著高于CC型个体.本研究初步表明IL-1β基因1 215多态位点可以作为抗病育种中的候选遗传标记.
本研究旨存通過檢測鷄白細胞介素-1β(interleukin-1β,IL-1β)基因部分區域單覈苷痠多態位點,以期查找與血清中IL-1含量相關的遺傳標記.根據紅色原鷄IL-1β基因序列設計2對引物,利用PCR及單鏈構象多態性(Single-Strand Conformation Polymorphism,SSCP)方法對如皋鷄、文昌鷄和安卡鷄進行單覈苷痠多態性(Single nucleotide polymorphism,SNP)分析,併利用試劑盒ELISA法對56日齡血清IL-1含量進行檢測.結果錶明血清中IL-1含量安卡鷄極顯著高于如皋鷄(P<0.01)和文昌鷄(P<0.001).IL-1β基因476位點髮生G→A突變、482位點髮生T→C突變、1 215位點髮生C→T突變、1 507位點髮生T→C突變.對多態位點和IL-1含量作關聯分析,隻髮現1 215位點與IL-1含量有關,TT基因型箇體IL-1含量顯著高于CC型箇體.本研究初步錶明IL-1β基因1 215多態位點可以作為抗病育種中的候選遺傳標記.
본연구지존통과검측계백세포개소-1β(interleukin-1β,IL-1β)기인부분구역단핵감산다태위점,이기사조여혈청중IL-1함량상관적유전표기.근거홍색원계IL-1β기인서렬설계2대인물,이용PCR급단련구상다태성(Single-Strand Conformation Polymorphism,SSCP)방법대여고계、문창계화안잡계진행단핵감산다태성(Single nucleotide polymorphism,SNP)분석,병이용시제합ELISA법대56일령혈청IL-1함량진행검측.결과표명혈청중IL-1함량안잡계겁현저고우여고계(P<0.01)화문창계(P<0.001).IL-1β기인476위점발생G→A돌변、482위점발생T→C돌변、1 215위점발생C→T돌변、1 507위점발생T→C돌변.대다태위점화IL-1함량작관련분석,지발현1 215위점여IL-1함량유관,TT기인형개체IL-1함량현저고우CC형개체.본연구초보표명IL-1β기인1 215다태위점가이작위항병육충중적후선유전표기.
The aim of the present study was to detect single nucleotide polymorphism (SNP) in some areas of IL-1β gene of chicken to find genetic marker influencing on IL-1 content in serum.Two pairs of primers were designed according to the sequence of IL-1β gene of red jungle fowls,and the SNP of Rugao chicken(RG), Wenchang chicken(WC), and Anka chicken(AK) were analysed by PCR-SSCP, meanwhile, the IL-1 content in serum were detected at 56 days with reagent kit by ELISA. The results showed that the IL-1 content in serum of AK was significantly higher than that of RG(P<0.01) and WC(P<0.001). The mutation were found at four sites in all three breeds, and they were mutation G→A at 476 site, mutation T→C at 482 site, mutation C→T at 1 215 site and mutation T→C at 1 507 site. Then, the correlation between polymorphic sites and content of IL-1 were analysed, and only 1 215 site had relationship with IL-1 content in serum, which showed that chicken with TT genotype had significantly higher IL-1 content in serum than those with CC genotype. This study indicated that the 1 215 polymorphic site could be a genetic marker for IL-1content in serum.
