植物遗传资源学报
植物遺傳資源學報
식물유전자원학보
JOURNAL OF PLANT GENETLC RESOURCES
2010年
3期
305-308
,共4页
杨国辉%杨欣明%王睿辉%李立会%高爱农%刘伟华
楊國輝%楊訢明%王睿輝%李立會%高愛農%劉偉華
양국휘%양흔명%왕예휘%리립회%고애농%류위화
小麦-冰草附加系%P染色体%多价体%多态性
小麥-冰草附加繫%P染色體%多價體%多態性
소맥-빙초부가계%P염색체%다개체%다태성
wheat-Agropyron addition lines%P chromosome%multivalent%polymorphism
在本研究室获得的一套小麦-冰草附加系材料中,附加了冰草(1·4)重组P染色体的附加系Ⅱ-21-2出现了较高频率的多价体.对小麦-冰草附加系Ⅱ-21-2的10个不同株系花粉母细胞减数分裂进行观察与统计,结果表明,不同株系均存在低频率的单价体,染色体异常联会主要表现为出现六价体和四价体,其中株系1-7-3出现六价体频率较高,出现六价体的花粉母细胞为41%,而株系1-7-7有13%的花粉母细胞出现四价体.小麦SSR标记分析表明,不同株系存在小麦基因组或P基因组的多态性.小麦一冰草附加系Ⅱ-21-2染色体异常联会可能与附加的P染色体有关,而其分子水平的多态性和重组可能对于遗传改良具有潜在的意义.
在本研究室穫得的一套小麥-冰草附加繫材料中,附加瞭冰草(1·4)重組P染色體的附加繫Ⅱ-21-2齣現瞭較高頻率的多價體.對小麥-冰草附加繫Ⅱ-21-2的10箇不同株繫花粉母細胞減數分裂進行觀察與統計,結果錶明,不同株繫均存在低頻率的單價體,染色體異常聯會主要錶現為齣現六價體和四價體,其中株繫1-7-3齣現六價體頻率較高,齣現六價體的花粉母細胞為41%,而株繫1-7-7有13%的花粉母細胞齣現四價體.小麥SSR標記分析錶明,不同株繫存在小麥基因組或P基因組的多態性.小麥一冰草附加繫Ⅱ-21-2染色體異常聯會可能與附加的P染色體有關,而其分子水平的多態性和重組可能對于遺傳改良具有潛在的意義.
재본연구실획득적일투소맥-빙초부가계재료중,부가료빙초(1·4)중조P염색체적부가계Ⅱ-21-2출현료교고빈솔적다개체.대소맥-빙초부가계Ⅱ-21-2적10개불동주계화분모세포감수분렬진행관찰여통계,결과표명,불동주계균존재저빈솔적단개체,염색체이상련회주요표현위출현륙개체화사개체,기중주계1-7-3출현륙개체빈솔교고,출현륙개체적화분모세포위41%,이주계1-7-7유13%적화분모세포출현사개체.소맥SSR표기분석표명,불동주계존재소맥기인조혹P기인조적다태성.소맥일빙초부가계Ⅱ-21-2염색체이상련회가능여부가적P염색체유관,이기분자수평적다태성화중조가능대우유전개량구유잠재적의의.
The high frequency of muhivalents was found in wheat-Agropyron addition line Ⅱ-21-2 which was Agropyron addition line Ⅱ-21-2 were observed.The result showed that there was low-frequency of univalent in all plants and the abnormal chromosome synapses mainly were s and quadrivalents.41% pollen-mother cells in 1-7-3 line hads,while 13% pollen-mother cells in 1-7-7 line had quadrivalents.The analysis of SSR primers from wheat showed that there was polymorphism in wheat genome or P genome of the different plants.Our results suggested that P chromosomes may influence the chromosome synapsis of wheat-Agropyron addition line Ⅱ-21-2 and the molecular polymorphism and recombination of wheat-Agropyron addition line Ⅱ-21-2 may be useful in genetic improvement of wheat.