CAJ | 학술논문

目的:观察黄酮对兔眼部血流量和大鼠脉络膜新生血管(CNV)的作用.方法:在体研究中,我们采用彩色微球技术测定黄酮对兔眼部血流量的影响.预先给予5g/L黄酮滴眼液(3次/d),1wk后用激光诱导大鼠CNV的生成,在2和4wk末,采用荧光血管造影(FA)测量CNV面积.离体研究中,采用MTT法检测黄酮对人脐静脉内皮细胞(HUVECs)存活率的影响.结果:黄酮显著的增加了兔眼部血流量,抑制了由激光诱导的CNV的生成.离体研究结果表明,黄酮抑制了HUVEC的增生.结论:黄酮能够增加眼部血流量,并且抑制CNV的生成.
목적:관찰황동대토안부혈류량화대서맥락막신생혈관(CNV)적작용.방법:재체연구중,아문채용채색미구기술측정황동대토안부혈류량적영향.예선급여5g/L황동적안액(3차/d),1wk후용격광유도대서CNV적생성,재2화4wk말,채용형광혈관조영(FA)측량CNV면적.리체연구중,채용MTT법검측황동대인제정맥내피세포(HUVECs)존활솔적영향.결과:황동현저적증가료토안부혈류량,억제료유격광유도적CNV적생성.리체연구결과표명,황동억제료HUVEC적증생.결론:황동능구증가안부혈류량,병차억제CNV적생성.
AIM:To investigate the effect of flavone on ocular blood flow in rabbit eyes and the formation of choroidal neovascularization(CNV)in rat model of age-related macular degeneration(AMD).METHODS:In in vivo studies,colored microsphere technique was used to determine the ocular blood flow in ocular hypertensive rabbit eyes.The rat eyes were treated with 5g/L flavone eye drops 3 times a day for 1 week before and 4 weeks after laser-induced injury of Bruch's membrane.The development of CNV was determined by fluorescein angiography(FA)performed on weeks 2 and 4.In in vitro studies,the effect of flavone on the viability of HUVECs was measured by MTT assay.RESULTS:The ocular blood flow in rabbit eyes was significantly increased after flavone instillation.Flavone significantly inhibited the formation of laser induced CNV.In vitro results showed that fiavone inhibited the proliferation of HUVECs.CONCLUSION:Flavone could increase ocular blood flow and inhibit the formation of CNV.