CAJ | 학술논문

目的在疑难配血中建立一种自动化、快速、准确的供血者标本血型抗原筛查方法.方法使用自动加样快速微孔板法对188个供血者全血标本进行C、e、M、Fya、Jka抗原检测,同时使用手工试管法进行平行实验;记录并对比上述两种方法筛查总过程使用的时间,筛查出的供者标本数.用微板法和试管法对抗-C、抗-e、抗-M、抗-Fya、抗-Jka试剂进行抗体效价测定.结果微孔板法、试管法均筛查出相同血型抗原阴性供者,微孔板筛查所需要的总时间较短.效价测定显示微板法灵敏度与手工试管法相同.结论微孔板法在疑难配血中筛查特定抗原阴性的供者,具有快速、准确的效果.
목적 재의난배혈중건립일충자동화、쾌속、준학적공혈자표본혈형항원사사방법.방법 사용자동가양쾌속미공판법대188개공혈자전혈표본진행C、e、M、Fya、Jka항원검측,동시사용수공시관법진행평행실험;기록병대비상술량충방법사사총과정사용적시간,사사출적공자표본수.용미판법화시관법대항-C、항-e、항-M、항-Fya、항-Jka시제진행항체효개측정.결과 미공판법、시관법균사사출상동혈형항원음성공자,미공판사사소수요적총시간교단.효개측정현시미판법령민도여수공시관법상동.결론 미공판법재의난배혈중사사특정항원음성적공자,구유쾌속、준학적효과.
Objective To establish a rapid, automatic and accurate method for screening the blood-group antigens in blood donors whose cross-match test are complicated. Methods The blood-group antigens of C ,e, M, Fya and Jka were determined by the automatic sampling microplate assay and test tube method parallelly in 188 blood donors, which the execution time and the number of suitable donors were recorded respectively. Meanwhile, the titers of anti-C, anti-e, anti-M, anti-Fya and anti-Jka antibodies were detected by those methods above-mentioned. Results The same blood donors with negative antigens could be identified through microplate assay and tube test method in both, but the forward method was more rapid. Moreover, the titrations of antibodies were consistent in microplate assay and tube test method. Conclusions We could consider that automatic sampling microplate assay is a rapid,accurate and available method for screening the blood-group antigens in blood donors' specimens whose cross-match test are complicated.