中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2008年
8期
1031-1033
,共3页
周凤珍%李明%高露露%周霞平%高素清%李佳倪%柯玮琳%罗军
週鳳珍%李明%高露露%週霞平%高素清%李佳倪%柯瑋琳%囉軍
주봉진%리명%고로로%주하평%고소청%리가예%가위림%라군
产前诊断/方法%穿刺术/方法
產前診斷/方法%穿刺術/方法
산전진단/방법%천자술/방법
Prenatal diagnosis/MT%Punetures/MT
目的 探讨胚外体腔穿刺术的实施方法 及进行产前诊断的可行性研究.方法 孕6～12周要求人工流产的孕妇58例,在人工流产前行胚外体腔穿刺术抽取胚外体腔液,采用聚合酶链反应(PCR)技术检测Y染色体上SRY(sex-determining resion Y)基因,并以绒毛检测结果 作为对照标准.观察穿刺前后胎心率、囊内出血及血肿情况.结果 胚外体腔穿刺总成功率96.6%.穿刺均在5 min内完成,一次穿刺成功率孕6周～者为25.0%,孕7～10周者为93.2%,孕～12周者为50.0%.穿刺前后胎心率无明显变化(P＞0.05).胚外体腔细胞SRY基因检测的准确率为91.1%(51/56).其中30例女胎胚外体腔细胞检测结果 全部与绒毛相符;21例绒毛检测为男胎者,有5例胚外体腔细胞结果 不相符,男胎的准确率为80.8%(21/26).结论 胚外体腔穿刺术的最佳时间为孕7～10周,其操作简单易行,成功率高,安全可靠.
目的 探討胚外體腔穿刺術的實施方法 及進行產前診斷的可行性研究.方法 孕6～12週要求人工流產的孕婦58例,在人工流產前行胚外體腔穿刺術抽取胚外體腔液,採用聚閤酶鏈反應(PCR)技術檢測Y染色體上SRY(sex-determining resion Y)基因,併以絨毛檢測結果 作為對照標準.觀察穿刺前後胎心率、囊內齣血及血腫情況.結果 胚外體腔穿刺總成功率96.6%.穿刺均在5 min內完成,一次穿刺成功率孕6週～者為25.0%,孕7～10週者為93.2%,孕～12週者為50.0%.穿刺前後胎心率無明顯變化(P＞0.05).胚外體腔細胞SRY基因檢測的準確率為91.1%(51/56).其中30例女胎胚外體腔細胞檢測結果 全部與絨毛相符;21例絨毛檢測為男胎者,有5例胚外體腔細胞結果 不相符,男胎的準確率為80.8%(21/26).結論 胚外體腔穿刺術的最佳時間為孕7～10週,其操作簡單易行,成功率高,安全可靠.
목적 탐토배외체강천자술적실시방법 급진행산전진단적가행성연구.방법 잉6～12주요구인공유산적잉부58례,재인공유산전행배외체강천자술추취배외체강액,채용취합매련반응(PCR)기술검측Y염색체상SRY(sex-determining resion Y)기인,병이융모검측결과 작위대조표준.관찰천자전후태심솔、낭내출혈급혈종정황.결과 배외체강천자총성공솔96.6%.천자균재5 min내완성,일차천자성공솔잉6주～자위25.0%,잉7～10주자위93.2%,잉～12주자위50.0%.천자전후태심솔무명현변화(P＞0.05).배외체강세포SRY기인검측적준학솔위91.1%(51/56).기중30례녀태배외체강세포검측결과 전부여융모상부;21례융모검측위남태자,유5례배외체강세포결과 불상부,남태적준학솔위80.8%(21/26).결론 배외체강천자술적최가시간위잉7～10주,기조작간단역행,성공솔고,안전가고.
Objective To explore the feasibility of coelocentesis in prenatal diagnosis.Methods Coelocentesis were applied on 58 women scheduled for at 6～12 weeks of gestation,and fetal heart rate before the procedure and 1,5,30 minutes afterwards and hemorrhage of amnionic cyst was observed.Y gene of sex-determining region was detected by polymerase chain reaction,and compared with chorionic villi sample.Results The total achievement ratio of coelocentesis was 96.6% of all cases in five minutes.The coelomic fluid was successfully aspirated at first attempt in 93.2% at 7～10 week's gestation,25.0% at 6～7weeks and 50.0% at 10 ~ 12 weeks.There was no significant difference in FHR between before coelocentesis and afterwards (P＞0.05).The detection accurate rate of SRY was 91.1%,all female fetus were matched with those observation by chorionic villi sampling,and only 5 male fetus were not matched,the accurate rate was 80.8%.Conclusion Coclocentesis has the advantage of simple procedure,safe and high feasibility.The optimal time of coclocentesis is in the 7th to 10th week of gestation.
