中华临床营养杂志
中華臨床營養雜誌
중화림상영양잡지
CHINESE JOURNAL OF CLINICAL NUTRITION
2012年
3期
143-148
,共6页
刘雪姣%李明喜%高碧霞%樊小红%蔡建芳%高友鹤%李雪梅%李学旺
劉雪姣%李明喜%高碧霞%樊小紅%蔡建芳%高友鶴%李雪梅%李學旺
류설교%리명희%고벽하%번소홍%채건방%고우학%리설매%리학왕
代谢综合征%尿蛋白质组%谱图数定量%蛋白功能分析
代謝綜閤徵%尿蛋白質組%譜圖數定量%蛋白功能分析
대사종합정%뇨단백질조%보도수정량%단백공능분석
Metabolic syndrome%Urinary proteomics%Spectral counting%Function analysis of proteins
目的 建立代谢综合征(MetS)患者尿液蛋白质组表达谱,对比MetS与正常人尿液差异蛋白,分析差异蛋白功能,以探讨MetS发病机制.方法 收集正常人和MetS患者过夜尿,丙酮沉淀法富集蛋白,将蛋白进行组内混合,反相液相色谱串联质谱鉴定蛋白,谱图数定量法定量蛋白,基因本体论分析方法进行差异蛋白功能分析.结果 正常人和MetS患者共鉴定到807和630个蛋白.MetS患者相比正常人上调蛋白有23个,其中纤溶酶原参与纤溶酶原激活连锁反应；37个下调蛋白中,α-烯醇酶、磷酸甘油激酶1和果糖二磷酸醛缩酶B分别参与糖酵解和果糖代谢过程.结论 建立了MetS患者尿蛋白质组表达谱,鉴定了MetS患者尿液差异蛋白.发现纤溶酶原激活连锁反应、糖酵解、果糖代谢异常可能在MetS发生发展过程中发挥重要作用.
目的 建立代謝綜閤徵(MetS)患者尿液蛋白質組錶達譜,對比MetS與正常人尿液差異蛋白,分析差異蛋白功能,以探討MetS髮病機製.方法 收集正常人和MetS患者過夜尿,丙酮沉澱法富集蛋白,將蛋白進行組內混閤,反相液相色譜串聯質譜鑒定蛋白,譜圖數定量法定量蛋白,基因本體論分析方法進行差異蛋白功能分析.結果 正常人和MetS患者共鑒定到807和630箇蛋白.MetS患者相比正常人上調蛋白有23箇,其中纖溶酶原參與纖溶酶原激活連鎖反應；37箇下調蛋白中,α-烯醇酶、燐痠甘油激酶1和果糖二燐痠醛縮酶B分彆參與糖酵解和果糖代謝過程.結論 建立瞭MetS患者尿蛋白質組錶達譜,鑒定瞭MetS患者尿液差異蛋白.髮現纖溶酶原激活連鎖反應、糖酵解、果糖代謝異常可能在MetS髮生髮展過程中髮揮重要作用.
목적 건립대사종합정(MetS)환자뇨액단백질조표체보,대비MetS여정상인뇨액차이단백,분석차이단백공능,이탐토MetS발병궤제.방법 수집정상인화MetS환자과야뇨,병동침정법부집단백,장단백진행조내혼합,반상액상색보천련질보감정단백,보도수정량법정량단백,기인본체론분석방법진행차이단백공능분석.결과 정상인화MetS환자공감정도807화630개단백.MetS환자상비정상인상조단백유23개,기중섬용매원삼여섬용매원격활련쇄반응；37개하조단백중,α-희순매、린산감유격매1화과당이린산철축매B분별삼여당효해화과당대사과정.결론 건립료MetS환자뇨단백질조표체보,감정료MetS환자뇨액차이단백.발현섬용매원격활련쇄반응、당효해、과당대사이상가능재MetS발생발전과정중발휘중요작용.
Objective To establish the urinary proteome profile of the metabolic syndrome ( MetS ) patients,compare the different urinary proteins between the MetS patients and the normal individuals,and analyze the function of the different proteins,so as to explore the pathogenesis of MetS.Methods Overnight urine were collected from normal controls (n =6) and MetS patients ( n =6).Acetone precipitation method was used to precipitate proteins of urine.Intra-group proteins were mixed together,identified by reversed phase liquid chromatography-mass spectrometry/mass spectrometry and quantified relatively using spectral counting method.The functions of differential proteins were analyzed using Panther.Results A total of 807 and 630 proteins were identified respectively in normal controls and MetS patients.Comparing MetS patients with normal controls,sixty different proteins were found,of which 23 proteins were up-regulated and 37 proteins were down-regulated in MetS patients.In the up-regulated proteins,plasminogen was involved in the plasminogen activation cascade and isoform of alphaenolase,phosphoglycerate kinase 1 and fructose-bisphosphate aldolase B down-regulated in MetS patients were involved in the process of glycolysis and fructose metabolism.Conclusions The urinary proteome profile of patients with MetS was established by reversed phase liquid chromatography-mass spectrometry/mass spectrometry.Different proteins between MetS patients and normal people were identified.The plasminogen activation cascade,glycolysis and fructose metabolism play key roles in the pathogenesis of MetS.
