CAJ | 학술논문

目的研究富勒烯化合物对斑马鱼受到γ射线照射后的存活率、畸形发生率的影响及其机制,以了解富勒烯类化合物对辐射生物效应的修饰作用.方法 ~(60)Co γ射线照射下富勒烯母体纳米颗粒对斑马鱼成鱼存活率的改变,同时利用斑马鱼胚胎-幼体比较该化合物对不同照射条件下斑马鱼鱼卵及幼体畸形发生率的影响,并从活性氧(ROS)生成的改变及DNA链断裂形成探讨了其可能的作用机制.结果光照期给药500×10~(-9)的富勒烯母体(nano-C_(60))能增强γ射线照射对成鱼的杀伤作用,在此条件下活性氧生成和DNA损伤增加,且抑制胚胎的孵出率和导致畸形的发生率增加;但避光期用药对射线效应没有明显的增强作用,除了高浓度5000×10~(-9)会抑制幼鱼的孵出率,并导致发育畸形增加.结论富勒烯母体对斑马鱼的辐射损伤效应有增强作用,其机制可能是在光照期用药后活化并导致活性氧生成增加,并导致DNA氧化损伤增强.
목적 연구부륵희화합물대반마어수도γ사선조사후적존활솔、기형발생솔적영향급기궤제,이료해부륵희류화합물대복사생물효응적수식작용.방법 ~(60)Co γ사선조사하부륵희모체납미과립대반마어성어존활솔적개변,동시이용반마어배태-유체비교해화합물대불동조사조건하반마어어란급유체기형발생솔적영향,병종활성양(ROS)생성적개변급DNA련단렬형성탐토료기가능적작용궤제.결과 광조기급약500×10~(-9)적부륵희모체(nano-C_(60))능증강γ사선조사대성어적살상작용,재차조건하활성양생성화DNA손상증가,차억제배태적부출솔화도치기형적발생솔증가;단피광기용약대사선효응몰유명현적증강작용,제료고농도5000×10~(-9)회억제유어적부출솔,병도치발육기형증가.결론 부륵희모체대반마어적복사손상효응유증강작용,기궤제가능시재광조기용약후활화병도치활성양생성증가,병도치DNA양화손상증강.
Objective To evaluate the modification of C_(60) on the radiation effects of ~(60)Co-γ irradiation on zebrafish.Methods The adult and embryonic zebrafish were used as model organisms to examine the potential of C_(60) to elicit oxidative stress responses on the surviving rate,hatching rate and malformation occurrence,both upon exposure to light or in the dark.Reactive oxygen species (ROS) production and DNA damage were examined as the possible underlying mechanism.Results 500 × 10~(-9) nano-C_(60) waterborne exposure could enhance the γ-irradiation effects by decreasing adult fish survival upon light exposure,which resulted in ROS and DNA damage increasing.The hatching rates were also inhibited with higher malformation,though dark exposure did not make any enhancement,except that the 5000× 10~(-9) C_(60) would inhibit larvae hatching and induced more malformation.Conclusions Waterborne nano-C_(60) exposure may enhance the radiation effects on zebrafish,ROS production and DNA damage increasing may be the underlying mechanism.