中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2012年
10期
742-746
,共5页
郭谦楠%廖世秀%康冰%张菊新%王睿丽%丁雪冰%张卫华
郭謙楠%廖世秀%康冰%張菊新%王睿麗%丁雪冰%張衛華
곽겸남%료세수%강빙%장국신%왕예려%정설빙%장위화
铁氧化还原蛋白NADP还原酶%流产,习惯性%多态现象,遗传%聚合酶链反应
鐵氧化還原蛋白NADP還原酶%流產,習慣性%多態現象,遺傳%聚閤酶鏈反應
철양화환원단백NADP환원매%유산,습관성%다태현상,유전%취합매련반응
Ferredoxin-NADP reductase%Abortion,habitual%Polymorphism,genetic%Polymerase chain reaction
目的 探讨蛋氨酸合成酶还原酶(MTRR) A66G基因多态性与不明原因复发性流产(URSA)的相关性.方法 采用PCR限制性片段长度多态性分析(PCR-RFLP)方法,对200对河南汉族URSA夫妇(URSA组)及76对有健康生育史的河南汉族夫妇(对照组)的基因型进行分析.结果 (1) MTRR A66G等位基因频率:MTRR A66G等位基因A和G的频率URSA组分别为男性76.5％(153/200)、女性72.8％ (146/200)；男性23.5％ (47/200)、女性27.2％ (54/200)；对照组分别为男性78.9％ (60/76)、女性78.3％ (59/76)；男性21.1％(16/76)、女性21.7％ (16/76).MTRR A66G等位基因频率在各组不同性别者及两组同性别者间比较,差异均无统计学意义(P＞0.05).(2) MTRR A66G基因型频率:MTRR A66G基因型AA、AG、GG频率URSA组分别为男性57.0％ (114/200)、女性52.0％(104/200)；男性39.0％ (78/200)、女性41.5％ (83/200)；男性4.0％(8/200)、女性6.5％(13/200)；对照组分别为男性59.2％(45/76)、女性59.2％(50/76)；男性39.5％(30/76)、女性38.2％ (29/76)；男性1.3％ (1/76)、女性2.6％ (2/76).MTRR A66G基因型频率在各组不同性别者及两组同性别者间比较,差异也无统计学意义(P＞0.05).(3)联合基因型频率:夫妇联合基因型GG+ GG、GG+AG、GG+AA、AG+ AG、AG+AA、AA+AA频率URSA组分别为1.0％(2/200)、2.5％ (5/200)、6.0％(12/200)、20.0％(40/200)、38.0％ (76/200)、32.5％ (65/200)；对照组分别为0、1.3％(1/76)、2.6％ (2/76)、17.1％ (13/76)、42.1％(32/76)、36.8％ (28/76)；两组夫妇联合基因型比较,差异也无统计学意义(P＞0.05).结论 MTRR A66G位点多态性与URSA的发生无明显相关性,不是URSA的遗传易感基因.
目的 探討蛋氨痠閤成酶還原酶(MTRR) A66G基因多態性與不明原因複髮性流產(URSA)的相關性.方法 採用PCR限製性片段長度多態性分析(PCR-RFLP)方法,對200對河南漢族URSA伕婦(URSA組)及76對有健康生育史的河南漢族伕婦(對照組)的基因型進行分析.結果 (1) MTRR A66G等位基因頻率:MTRR A66G等位基因A和G的頻率URSA組分彆為男性76.5％(153/200)、女性72.8％ (146/200)；男性23.5％ (47/200)、女性27.2％ (54/200)；對照組分彆為男性78.9％ (60/76)、女性78.3％ (59/76)；男性21.1％(16/76)、女性21.7％ (16/76).MTRR A66G等位基因頻率在各組不同性彆者及兩組同性彆者間比較,差異均無統計學意義(P＞0.05).(2) MTRR A66G基因型頻率:MTRR A66G基因型AA、AG、GG頻率URSA組分彆為男性57.0％ (114/200)、女性52.0％(104/200)；男性39.0％ (78/200)、女性41.5％ (83/200)；男性4.0％(8/200)、女性6.5％(13/200)；對照組分彆為男性59.2％(45/76)、女性59.2％(50/76)；男性39.5％(30/76)、女性38.2％ (29/76)；男性1.3％ (1/76)、女性2.6％ (2/76).MTRR A66G基因型頻率在各組不同性彆者及兩組同性彆者間比較,差異也無統計學意義(P＞0.05).(3)聯閤基因型頻率:伕婦聯閤基因型GG+ GG、GG+AG、GG+AA、AG+ AG、AG+AA、AA+AA頻率URSA組分彆為1.0％(2/200)、2.5％ (5/200)、6.0％(12/200)、20.0％(40/200)、38.0％ (76/200)、32.5％ (65/200)；對照組分彆為0、1.3％(1/76)、2.6％ (2/76)、17.1％ (13/76)、42.1％(32/76)、36.8％ (28/76)；兩組伕婦聯閤基因型比較,差異也無統計學意義(P＞0.05).結論 MTRR A66G位點多態性與URSA的髮生無明顯相關性,不是URSA的遺傳易感基因.
목적 탐토단안산합성매환원매(MTRR) A66G기인다태성여불명원인복발성유산(URSA)적상관성.방법 채용PCR한제성편단장도다태성분석(PCR-RFLP)방법,대200대하남한족URSA부부(URSA조)급76대유건강생육사적하남한족부부(대조조)적기인형진행분석.결과 (1) MTRR A66G등위기인빈솔:MTRR A66G등위기인A화G적빈솔URSA조분별위남성76.5％(153/200)、녀성72.8％ (146/200)；남성23.5％ (47/200)、녀성27.2％ (54/200)；대조조분별위남성78.9％ (60/76)、녀성78.3％ (59/76)；남성21.1％(16/76)、녀성21.7％ (16/76).MTRR A66G등위기인빈솔재각조불동성별자급량조동성별자간비교,차이균무통계학의의(P＞0.05).(2) MTRR A66G기인형빈솔:MTRR A66G기인형AA、AG、GG빈솔URSA조분별위남성57.0％ (114/200)、녀성52.0％(104/200)；남성39.0％ (78/200)、녀성41.5％ (83/200)；남성4.0％(8/200)、녀성6.5％(13/200)；대조조분별위남성59.2％(45/76)、녀성59.2％(50/76)；남성39.5％(30/76)、녀성38.2％ (29/76)；남성1.3％ (1/76)、녀성2.6％ (2/76).MTRR A66G기인형빈솔재각조불동성별자급량조동성별자간비교,차이야무통계학의의(P＞0.05).(3)연합기인형빈솔:부부연합기인형GG+ GG、GG+AG、GG+AA、AG+ AG、AG+AA、AA+AA빈솔URSA조분별위1.0％(2/200)、2.5％ (5/200)、6.0％(12/200)、20.0％(40/200)、38.0％ (76/200)、32.5％ (65/200)；대조조분별위0、1.3％(1/76)、2.6％ (2/76)、17.1％ (13/76)、42.1％(32/76)、36.8％ (28/76)；량조부부연합기인형비교,차이야무통계학의의(P＞0.05).결론 MTRR A66G위점다태성여URSA적발생무명현상관성,불시URSA적유전역감기인.
Objective To explore the relationship between the polymorphism of methionine synthase reductase(MTRR) A66G and the susceptibility to unexplained repeated spontaneous abortion (URSA).Methods Total of 200 Henan Han couples with URSA (URSA group) and 76 Henan Han healthy couples without URSA (control group)were enrolled in this study.Their MTRR A66G genotypes were determined by PCR restriction fragment length polymorphism (PCR-RFLP).Results (1) The allele frequencies of MTRR A66G:the frequencies of allele A and allele G in URSA group were 76.5％ (153/200)in husband and 72.8％ (146/200) in wife,23.5％ (47/200) in husband and 27.2％ (54/200) in wife,respectively.The frequencies of allele A and allele G in control group were 78.9％ (60/76) in husband and 78.3％ (59/76) in wife,21.1％ (16/76) in husband and 21.7％ (16/76) in wife,respectively.The frequencies of allele A and allele G were not significantly different between female and male subjects within the same experimental group (P ＞ 0.05),and also there were not significantly different between the same gender subjects at URAS and control groups(P ＞ 0.05).(2) The genotype frequencies of MTRR A66G:the frequencies of genotype AA,AG and GG in URSA group were 57.0％ (114/200) in husband and 52.0％ (104/200) in wife,39.0％ (78/200) in husband and 41.5％ (83/200) in wife,4.0％ (8/200) in husband and 6.5％ (13/200) in wife,prepectively.The frequencies of genotype AA,AG and GG in control group were 59.2％ (45/76) in husband and 59.2％ (50/76) in wife,39.5％ (30/76) in husband and 38.2％ (29/76) in wife;1.3 ％ (1/76) in husband and 2.6％ (2/76) in wife,prepectively.The frequencies of genotype AA,AG and GG were not significantly different between female and male subjects within the same group (P ＞ 0.05),and also there were not significantly different between the same gender subjects at URSA and control groups (P ＞0.05).(3) Combined genotype of couples:the combined genotype frequencies of GG + GG,GG + AG,GG +AA,AG + AG,AG + AA and AA + AA in URSA group were 1.0％ (2/200),2.5％ (5/200),6.0％ (12/200),20.0％ (40/200),38.0％ (76/200),and 32.5 ％ (65/200),prepectively ; the combined genotype frequencies in control group were 0,1.3％ (1/76),2.6％ (2/76),17.1％ (13/76),42.1％ (32/76),36.8％ (28/76),prepectively.The combined genotype analysis between the two groups were also not significantly different (P ＞ 0.05).Conclusion The polymorphism of MTRR A66G gene was not associated with the susceptibility to URSA (P ＞ 0.05),and so it was not the inherited genetic risk factor of URSA.
