CAJ | 학술논문

目的研究磷酸化蛋白激酶B(p-AKT)、磷酸化糖原合成酶激酶-3β( p-GSK3β)和β-catenin在卵巢上皮性肿瘤中的表达,探讨其与卵巢上皮性肿瘤临床病理特征的关系.方法采用免疫组织化学EnVision二步法检测10例卵巢良性上皮性肿瘤、10例卵巢交界性上皮性肿瘤及70例上皮性卵巢癌中p-AKT、p-GSK3β及β-catenin的表达,并分析它们的相关性及与临床病理特征的关系.结果 (1)卵巢癌组织中p-AKT、p-GSK3β及β-catenin的阳性表达率分别为67.1％( 47/70)、60.0％ (42/70)和71.4％ (50/70),三者在卵巢癌组的表达水平与其他两组相比,差异均有统计学意义(均P＜0.05).p-AKT与p-GSK3β、p-GSK3β与β-catenin、p-AKT与β-catenin在卵巢癌中的表达均呈正相关(r值分别为0.546、0.581、0.500;P值均＜0.05).(2)p-AKT在上皮性卵巢癌组织中的表达水平与交界性和良性肿瘤相比,差异均有统计学意义(均P ＜0.05).p-AKT与卵巢癌的分化程度密切相关(P＜0.05),而与卵巢癌的组织学分型及临床分期无统计学相关性(P＞0.05).(3)p-GSK3β和β-catenin在卵巢癌中的表达水平均高于交界性和良性肿瘤,差异有统计学意义(均P＜0.05)；且其表达与卵巢癌的分化程度及临床分期密切相关(均P ＜0.05),而与卵巢癌的组织学分型无统计学相关性(均P＞0.05).结论在卵巢癌组织中p-AKT、p-GSK3β和β-catenin蛋白的表达具有正相关性,p-AKT结合p-GSK33并使其失活可能是导致卵巢癌中β-catenin活化的一个因素.
목적 연구린산화단백격매B(p-AKT)、린산화당원합성매격매-3β( p-GSK3β)화β-catenin재란소상피성종류중적표체,탐토기여란소상피성종류림상병리특정적관계.방법 채용면역조직화학EnVision이보법검측10례란소량성상피성종류、10례란소교계성상피성종류급70례상피성란소암중p-AKT、p-GSK3β급β-catenin적표체,병분석타문적상관성급여림상병리특정적관계.결과 (1)란소암조직중p-AKT、p-GSK3β급β-catenin적양성표체솔분별위67.1％( 47/70)、60.0％ (42/70)화71.4％ (50/70),삼자재란소암조적표체수평여기타량조상비,차이균유통계학의의(균P＜0.05).p-AKT여p-GSK3β、p-GSK3β여β-catenin、p-AKT여β-catenin재란소암중적표체균정정상관(r치분별위0.546、0.581、0.500;P치균＜0.05).(2)p-AKT재상피성란소암조직중적표체수평여교계성화량성종류상비,차이균유통계학의의(균P ＜0.05).p-AKT여란소암적분화정도밀절상관(P＜0.05),이여란소암적조직학분형급림상분기무통계학상관성(P＞0.05).(3)p-GSK3β화β-catenin재란소암중적표체수평균고우교계성화량성종류,차이유통계학의의(균P＜0.05)；차기표체여란소암적분화정도급림상분기밀절상관(균P ＜0.05),이여란소암적조직학분형무통계학상관성(균P＞0.05).결론 재란소암조직중p-AKT、p-GSK3β화β-catenin단백적표체구유정상관성,p-AKT결합p-GSK33병사기실활가능시도치란소암중β-catenin활화적일개인소.
Objective To investigate the expressions of phosphorylated protein kinase B (p-AKT),phosphorylated glycogen synthase kinase 3β (p-GSK3β) and β-catenin proteins and to evaluate their relationship with the clinical pathological characteristics in epithelial tumors of the ovary.Methods The expression of p-AKT,p-GSK3β,and β-catenin was detected with immunohistochemical staining ( EnVision method) in 10 cases of benign epithelial neoplasia,10 cases of borderline epithelial neoplasia and 70 cases of ovarian carcinoma.The relationship of the expression of p-AKT,p-GSK3β and β-catenin with the clinical pathological features was analyzed. Results The positive expression rates of p-AKT, p-GSK3β and β-catenin in epithelial ovarian carcinoma were 67.1％ (47/70),60.0％ (42/70) and 71.4％ (50/70),respectively.Compared to the results of benign and borderline epithelial neoplasia,the expression of the three proteins in carcinoma of the ovary was significantly different ( all P ＜ 0.05 ).Positive correlation was found between p-AKT and p-GSK3β,p-GSK3β and β-catenin,and p-AKT and β-catenin in epithelial ovarian carcinoma ( r =0.546,0.581,0.500,respectively; all P ＜ 0.05 ).Compared to the results of benign and borderline epithelial neoplasia,the expression of p-AKT protein in epithelial ovarian carcinoma was significantly different ( all P ＜ 0.05).The expression of p-AKT was correlated with the differentiation of epithelial ovarian carcinoma ( P ＜ 0.05 ), but no relationship was found between its expression and histological classification and FIGO staging ( P ＞ 0.05 ).The expression of p-GSK3β and β-catenin in epithelial ovarian carcinoma were both higher than that in benign and borderline epithelial neoplasia ( P ＜ 0.05 ),and correlated with tumor differentiation and FIGO staging ( P ＜ 0.05 ),but no relationship were found between their expression with histological classification ( P ＞ 0.05 ). Conclusions Positive correlations are found between p-AKT, p-GSK3β and β-catenin in epithelial ovarian carcinoma. The activation of β-catenin is possibly correlated with inactivation of p-GSK3β that binds to p-AKT.