CAJ | 학술논문

目的探讨SLE患者外周血B细胞凋亡抑制蛋白c-FLIP的表达及其与临床特征的相关性.方法 53例SLE患者和30例正常人对照组,采用流式细胞术检测外周血B细胞c-FLIP表达阳性率,采用ELISA方法检测血清中IL-4和IL-10的水平.结果活动期SLE患者(18例)外周血B细胞内c-FLIP表达(3.11%±0.70%)明显高于非活动期患者(35例)(0.78%±0.28%)和正常人对照组(0.68%±0.12%)(P均＜0.05),而非活动期组和正常人对照组比较,差异无统计学意义(t=1.56,P＞0.05).SLE患者外周血B细胞c-FLIP的表达与SLEDAI、ESR、C反应蛋白、ANA滴度均呈正相关(P均＜0.05);伴有WBC减少的36例SLE患者中c-FLIP的表达与WBC的数目呈负相关(P＜0.01).伴有狼疮肾炎的23例SLE患者的外周血B细胞内c-FLIP的表达(3.04%±1.09%)明显高于30例不伴狼疮.肾炎者(1.76%±1.09%)(t=4.23,P＜0.05).SLE患者c-FLIP的表达与血清中IL-4和IL-10的水平均呈正相关(P均＜0.01).结论活动期SLE患者外周血B细胞c-FLIP高表达,且与SLE病情严重程度正相关.同时其表达水平与SLE患者血清中IL-4和IL-10水平呈正相关.
목적 탐토SLE환자외주혈B세포조망억제단백c-FLIP적표체급기여림상특정적상관성.방법 53례SLE환자화30례정상인대조조,채용류식세포술검측외주혈B세포c-FLIP표체양성솔,채용ELISA방법검측혈청중IL-4화IL-10적수평.결과 활동기SLE환자(18례)외주혈B세포내c-FLIP표체(3.11%±0.70%)명현고우비활동기환자(35례)(0.78%±0.28%)화정상인대조조(0.68%±0.12%)(P균＜0.05),이비활동기조화정상인대조조비교,차이무통계학의의(t=1.56,P＞0.05).SLE환자외주혈B세포c-FLIP적표체여SLEDAI、ESR、C반응단백、ANA적도균정정상관(P균＜0.05);반유WBC감소적36례SLE환자중c-FLIP적표체여WBC적수목정부상관(P＜0.01).반유랑창신염적23례SLE환자적외주혈B세포내c-FLIP적표체(3.04%±1.09%)명현고우30례불반랑창.신염자(1.76%±1.09%)(t=4.23,P＜0.05).SLE환자c-FLIP적표체여혈청중IL-4화IL-10적수평균정정상관(P균＜0.01).결론 활동기SLE환자외주혈B세포c-FLIP고표체,차여SLE병정엄중정도정상관.동시기표체수평여SLE환자혈청중IL-4화IL-10수평정정상관.
Objective To study the expression of cellular FLICE inhibitory proteins(c-FLIP)in peripheral blood B lymphocytes in patients with systemic lupus erythematosus (SLE)and its correlation with clinical features.Methods Blood samples were obtained from 53 patients with SLE and 30 normal human controls.Flow cytometry and ELISA were performed to measure the expression of c-FLIP in pefipheral blood B lymphocytes and serum levels of IL-4 and IL-10,respectively.Relevant laboratory examinations were carried out for these patients.SLE disease activity index(SLEDAI)score was calculated for patients.Results The positivity rate of c-FLIP in B lymphocytes was 3.11%±0.70%in 18 patients with active SLE.significantly higher than that in 35 patients with inactive SLE (0.78%±0.28%)and normaI controls(0.68%±0.12%),while no statistical difierence was found between inactive patients and controls(t=1.56,P＞0.05).In SLE patients,the expression of c-FLIP showed a positive correlation with SLEDAl score(r=0.96.P＜0.05),erythrocyte sedimentation rate(r=0.96,P＜0.01),serum level of C reactive protein(r=0.92.P＜0.01)and the titer of antinuclear antibodies(r=0.86,P＜0.01),whereas in 36 patients with leucopenia.a negative correlation was noticed between white blood cell count and the expression level of c-FLIP(r=-0.94,P＜0.0 1).The 23 patients with lupus nephritis had a higher level of c-FLIP than those without lupus nephritis(3.04%±1.09%vs 1.76%±1.09%,t=4.23,P＜0.05).Additionally.the expression of c-FLIP positively correlated with the serum level of IL-4 and IL-10(r=0.80,0.89.respectively,both P＜0.01).Conclusions In patients with active SLE,the expression of c-FLIP is upregulated in peripheral blood B lymphocytes,and positively correlated with the severity of SLE as well as the serum level of IL-4 and IL-10.The upregulation of c-FLIP in B cells might play a certain role in deficient apoptosis or clearance of activated B cells in SLE.