CAJ | 학술논문

目的通过检测亚低温治疗后重型颅脑损伤患者血清S-100B蛋白含量的变化来证实亚低温治疗的脑保护作用,探讨其可能的作用机制.方法选取100例正常体检者为对照组,选取100例重型颅脑损伤患者(GCS≤8分1并分为亚低温治疗组50例和常温治疗组50例,分别于伤后早期(2～6 h)及伤后不同时间(1 d、3 d、5 d、7 d、10 d)采血,检测血清中S-100B蛋白含量,比较其在伤后不同时期的血清S-100B蛋白含量.结果正常体检者血清S-100B蛋白含量测定结果证实,正常人血清S-100B蛋白含量与年龄、性别无关.亚低温治疗组、常温治疗组患者伤后血清S-100B蛋白含量与对照组相比差异有统计学意义(P<0.01).伤后1 d、3 d、5 d、7 d、10 d时亚低温治疗组血清S-100B含量明显低于常温治疗组,差异有统计学意义(P(0.05).结论血清S-100B蛋白在重型颅脑损伤的诊断巾有高度敏感性和特异性,是一种有效的生化指标.亚低温治疗对重型颅脑损伤具有脑保护作用.
목적 통과검측아저온치료후중형로뇌손상환자혈청S-100B단백함량적변화래증실아저온치료적뇌보호작용,탐토기가능적작용궤제.방법 선취100례정상체검자위대조조,선취100례중형로뇌손상환자(GCS≤8분1병분위아저온치료조50례화상온치료조50례,분별우상후조기(2～6 h)급상후불동시간(1 d、3 d、5 d、7 d、10 d)채혈,검측혈청중S-100B단백함량,비교기재상후불동시기적혈청S-100B단백함량.결과 정상체검자혈청S-100B단백함량측정결과증실,정상인혈청S-100B단백함량여년령、성별무관.아저온치료조、상온치료조환자상후혈청S-100B단백함량여대조조상비차이유통계학의의(P<0.01).상후1 d、3 d、5 d、7 d、10 d시아저온치료조혈청S-100B함량명현저우상온치료조,차이유통계학의의(P(0.05).결론 혈청S-100B단백재중형로뇌손상적진단건유고도민감성화특이성,시일충유효적생화지표.아저온치료대중형로뇌손상구유뇌보호작용.
Objective To evaluate the effect of mild hypothermia for brain protection by detecting the changes in serum S-100B protein in patients with severe head injury and investigate the possible mechanism. Methods With 100 healthy volunteers as the control group, 100 patients with severe head injury (Glascow Coma Score ≤ 8) received treatment with mild hypothermia (n=50) or normothermia (n=50). Serum samples were collected from the patients early (2-6 h) after the injury and at 1, 3, 5, 7, and 10 days after the injury to measure the levels of S-100B protein. Results In healthy subjects, the serum level of S-100B protein was not associated with age or gender. Patients with severe head injury receiving mild hypothermia treatment and normothermia treatment showed significant differences in serum S-100B protein level from the control group (P<0.01). The patinets with mild hypothermia treatment had significantly lower serum S-100B level than those treated with normothermia atl, 3, 5, 7, and l0 days after the injury (P<0.05). Conclusion Serum S-100B protein has high sensitivity and specificity in the diagnosis of severe head injury, and may serve as an effective biochemical indicator. Mild hypothermia may protect the brain tissue of patients with severe head injury.