中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
10期
1761-1763
,共3页
林善昌%郑宝石%何巍%刘玳瑄%蔡旭东%刘与友%刘翔%周绍昉
林善昌%鄭寶石%何巍%劉玳瑄%蔡旭東%劉與友%劉翔%週紹昉
림선창%정보석%하외%류대선%채욱동%류여우%류상%주소방
心脏移植%氧合血%灌注%缺血再灌注损伤%急性排斥反应
心髒移植%氧閤血%灌註%缺血再灌註損傷%急性排斥反應
심장이식%양합혈%관주%결혈재관주손상%급성배척반응
Heart transplant%Oxygenated blood%Perfusion%Ischemic reperfusion injury%Acute rejection
目的 观察持续氧合血灌注供心对移植心脏急性排斥反应的影响.方法 建立无缺血再灌注损伤(同系A1组、异系B1组)和缺血再灌注损伤(同系A2组、异系B2组)4种异位心脏移植模型,并设立对照组C,术后第7天取标本,Real-Time聚合酶链反应(PCR)检测移植心脏MHC-Ⅱ、B7-2 mRNA表达,免疫组织化学法检测CD4+T细胞浸润.结果 A2、A1组MHC-ⅡmRNA分别为9.30±0.53、1.48 ±0.31 (P ＜0.05)、B7-2 mRNA分别为5.45±0.43、1.46 ±0.30(P ＜0.05)、CD4+T 细胞数分别为4.86±0.90、3.14 ±0.69(P＜0.05)；B2、B1组MHC-ⅡmRNA分别为21.12±1.15、14.14±0.80(P ＜0.05)、B7-2 mRNA分别为13.41±0.43、8.23±0.48(P ＜0.05)、CD4+T细胞数分别为43.57±2.07、18.86±0.69(P ＜0.05).结论 持续氧合血灌注供心有助于减轻或延缓急性排斥反应的发生.
目的 觀察持續氧閤血灌註供心對移植心髒急性排斥反應的影響.方法 建立無缺血再灌註損傷(同繫A1組、異繫B1組)和缺血再灌註損傷(同繫A2組、異繫B2組)4種異位心髒移植模型,併設立對照組C,術後第7天取標本,Real-Time聚閤酶鏈反應(PCR)檢測移植心髒MHC-Ⅱ、B7-2 mRNA錶達,免疫組織化學法檢測CD4+T細胞浸潤.結果 A2、A1組MHC-ⅡmRNA分彆為9.30±0.53、1.48 ±0.31 (P ＜0.05)、B7-2 mRNA分彆為5.45±0.43、1.46 ±0.30(P ＜0.05)、CD4+T 細胞數分彆為4.86±0.90、3.14 ±0.69(P＜0.05)；B2、B1組MHC-ⅡmRNA分彆為21.12±1.15、14.14±0.80(P ＜0.05)、B7-2 mRNA分彆為13.41±0.43、8.23±0.48(P ＜0.05)、CD4+T細胞數分彆為43.57±2.07、18.86±0.69(P ＜0.05).結論 持續氧閤血灌註供心有助于減輕或延緩急性排斥反應的髮生.
목적 관찰지속양합혈관주공심대이식심장급성배척반응적영향.방법 건립무결혈재관주손상(동계A1조、이계B1조)화결혈재관주손상(동계A2조、이계B2조)4충이위심장이식모형,병설립대조조C,술후제7천취표본,Real-Time취합매련반응(PCR)검측이식심장MHC-Ⅱ、B7-2 mRNA표체,면역조직화학법검측CD4+T세포침윤.결과 A2、A1조MHC-ⅡmRNA분별위9.30±0.53、1.48 ±0.31 (P ＜0.05)、B7-2 mRNA분별위5.45±0.43、1.46 ±0.30(P ＜0.05)、CD4+T 세포수분별위4.86±0.90、3.14 ±0.69(P＜0.05)；B2、B1조MHC-ⅡmRNA분별위21.12±1.15、14.14±0.80(P ＜0.05)、B7-2 mRNA분별위13.41±0.43、8.23±0.48(P ＜0.05)、CD4+T세포수분별위43.57±2.07、18.86±0.69(P ＜0.05).결론 지속양합혈관주공심유조우감경혹연완급성배척반응적발생.
Objective To study the effect of continuous perfusion of donor heart with oxygenated blood on acute rejection of the transplanted hearts.Methods Four kinds of rattish heterotopic cardiac transplantation models were established,two were no IRI models ( Group A1 for homogeneity and Group B1 for allogene) and the other two were IRI models (Group A2 for homogeneity and Group B1 for allogene).A blank control group C was also set up.Specimens were collected 7 days after operation.The levels of expressing MHC-2 mRNA and B7-2 mRNA were detected by Real-Time polymerase chain reaction (PCR).Immunohistochemical stain was applied to observe the CD4 + T cell infiltrating.Results For group A2 and A1,the levels of MHC-2 mRNA was respectively 9.30 ± 0.53,1.48 ± 0.31 ( P ＜ 0.05 ),that of B7-2 mRNA was respectively 5.45 ± 0.43,1.46 ± 0.30 ( P ＜ 0.05 ),and the number of CD4 + T cell was respectively 4.86 ±0.90,3.14 ±0.69(P ＜0.05) ; For group B2 and B1,the levels of MHC-2 mRNA was respectively 21.12 ± 1.15,14.14 ± 0.80 ( P ＜ 0.05 ),that of B7-2 mRNA was respectively 13.41 ±0.43,8.23 ± 0.48 ( P ＜ 0.05 ),and the number of CD4 + T cell was respectively 43.57 ± 2.07,18.86 ±0.69 (P ＜ 0.05).Conclusion It is beneficial to reduce the intensity or postpone occurrence of acute rejection after transplantation because of continuous perfusion of donor heart with oxygenated blood.
