中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2009年
7期
760-763
,共4页
张洋%李永哲%冯雪%包书萌%胡朝军%佟大伟%张蜀澜%张奉春
張洋%李永哲%馮雪%包書萌%鬍朝軍%佟大偉%張蜀瀾%張奉春
장양%리영철%풍설%포서맹%호조군%동대위%장촉란%장봉춘
肝硬化,胆汁性%自身抗体%免疫印迹法
肝硬化,膽汁性%自身抗體%免疫印跡法
간경화,담즙성%자신항체%면역인적법
Liver cirrhosis,biliary%Autoantibodies%Immunoblotting
目的 探讨联合检测多种自身抗体在原发性胆汁性肝硬化(PBC)诊断中的价值及临床意义.方法 用免疫印迹法分别检测96例PBC患者,100例其他自身免疫性疾病(AID)患者和49名健康体检者血清中的抗线粒体(AMA)-M2亚型抗体、抗3E(BPO)抗体、抗Sp100抗体、抗旱幼粒细胞性白血病(PML)抗体、抗gp210抗体、抗肝肾微粒体-1(LKM-1)抗体、抗肝特异性胞质抗体-1(LC-1)、抗可溶性肝抗原/肝胰抗原抗体(SLA/LP).结果 96例PBC患者中AMA-M2、抗3E(BPO)抗体、抗Sp100抗体、抗PML抗体、抗gp210抗体阳性率分别为76.0%、84.4%、32.3%、28.1%、35.4%,而以上5种自身抗体在其他自身免疫性疾病中的阳性率分别为13.0%、9.0%、3.0%、2.0%、1.0%;AMA-M2对PBC诊断的敏感度达76.0%,特异度达87.0%;抗3E(BPO)抗体对PBC诊断的敏感度达84.4%,特异度达91.0%;抗Sp100抗体对PBC诊断的敏感度达32.3%,特异度达97.0%;抗PML抗体对PBC诊断的敏感度达28.1%,特异度达98.%;抗gp210抗体对PBC诊断的敏感度达35.4%,特异度达99.0%,未检测到抗LKM-1抗体、抗LC-1抗体及抗SLA/LP抗体;抗gp210抗体阳性患者肝功能衰竭的发生率明显高于抗体阴性患者(χ2 =11.17,P<0.01).结论 PBC患者中可检测到多种自身抗体,自身免疫性肝病自身抗体谱检测对PBC诊断、鉴别诊断、治疗及预后判断均有重要意义.
目的 探討聯閤檢測多種自身抗體在原髮性膽汁性肝硬化(PBC)診斷中的價值及臨床意義.方法 用免疫印跡法分彆檢測96例PBC患者,100例其他自身免疫性疾病(AID)患者和49名健康體檢者血清中的抗線粒體(AMA)-M2亞型抗體、抗3E(BPO)抗體、抗Sp100抗體、抗旱幼粒細胞性白血病(PML)抗體、抗gp210抗體、抗肝腎微粒體-1(LKM-1)抗體、抗肝特異性胞質抗體-1(LC-1)、抗可溶性肝抗原/肝胰抗原抗體(SLA/LP).結果 96例PBC患者中AMA-M2、抗3E(BPO)抗體、抗Sp100抗體、抗PML抗體、抗gp210抗體暘性率分彆為76.0%、84.4%、32.3%、28.1%、35.4%,而以上5種自身抗體在其他自身免疫性疾病中的暘性率分彆為13.0%、9.0%、3.0%、2.0%、1.0%;AMA-M2對PBC診斷的敏感度達76.0%,特異度達87.0%;抗3E(BPO)抗體對PBC診斷的敏感度達84.4%,特異度達91.0%;抗Sp100抗體對PBC診斷的敏感度達32.3%,特異度達97.0%;抗PML抗體對PBC診斷的敏感度達28.1%,特異度達98.%;抗gp210抗體對PBC診斷的敏感度達35.4%,特異度達99.0%,未檢測到抗LKM-1抗體、抗LC-1抗體及抗SLA/LP抗體;抗gp210抗體暘性患者肝功能衰竭的髮生率明顯高于抗體陰性患者(χ2 =11.17,P<0.01).結論 PBC患者中可檢測到多種自身抗體,自身免疫性肝病自身抗體譜檢測對PBC診斷、鑒彆診斷、治療及預後判斷均有重要意義.
목적 탐토연합검측다충자신항체재원발성담즙성간경화(PBC)진단중적개치급림상의의.방법 용면역인적법분별검측96례PBC환자,100례기타자신면역성질병(AID)환자화49명건강체검자혈청중적항선립체(AMA)-M2아형항체、항3E(BPO)항체、항Sp100항체、항한유립세포성백혈병(PML)항체、항gp210항체、항간신미립체-1(LKM-1)항체、항간특이성포질항체-1(LC-1)、항가용성간항원/간이항원항체(SLA/LP).결과 96례PBC환자중AMA-M2、항3E(BPO)항체、항Sp100항체、항PML항체、항gp210항체양성솔분별위76.0%、84.4%、32.3%、28.1%、35.4%,이이상5충자신항체재기타자신면역성질병중적양성솔분별위13.0%、9.0%、3.0%、2.0%、1.0%;AMA-M2대PBC진단적민감도체76.0%,특이도체87.0%;항3E(BPO)항체대PBC진단적민감도체84.4%,특이도체91.0%;항Sp100항체대PBC진단적민감도체32.3%,특이도체97.0%;항PML항체대PBC진단적민감도체28.1%,특이도체98.%;항gp210항체대PBC진단적민감도체35.4%,특이도체99.0%,미검측도항LKM-1항체、항LC-1항체급항SLA/LP항체;항gp210항체양성환자간공능쇠갈적발생솔명현고우항체음성환자(χ2 =11.17,P<0.01).결론 PBC환자중가검측도다충자신항체,자신면역성간병자신항체보검측대PBC진단、감별진단、치료급예후판단균유중요의의.
Objective To detect the value of autoantibedy profile in primary biliary cirrhosis (PBC). Methods 96 serum samples of patients with primiary biliary cirrhosis, 100 serum samples of other antoimmune disease and 49 serum samples of healthy were tested for anti- M2, anti-3E(BPO), anti-Sp100,anti-PML,anti-gp210,anti-LKM-1 ,anti-LC-1 ,anti-SLA/LP by EUROLine. Results The positive of the anti-M2,anti-BPO, anti-Sp100, anti-PML and anti-gp210 for PBC was 76. 0%, 84.4%, 32. 3%, 28. 1% and 35.4% ,respectively. The positive of the anti-M2, anti-BPO, anti-Sp100, anti-PML and anti-gp210 for other autoimmune disease was 13.0% ,9. 0% ,3.0% ,2.0% and 1. 0%, respectively. The sensitivity of the anti-M2 for PBC was 76. 0% ,with specificity of 87. 0%. The sensitivity of the anti-BPO for PBC was 84. 4%, with specificity of 91.0% ;the sensitivity of the anti-Sp100 for PBC was 32. 3%, with specificity of 97.0%. The sensitivity of the anti-PML for PBC was 28. 1% ,with specificity of 98.0%. The sensitivity of the anti-gp210 for PBC was 35.4%, with specificity of 99. 0% . Anti-LKM-1, anti-LC-1, anti-SLA/LP positive patients with PBC were not detected;the incidence rate of liver function failure in anti-gp210 positive serum higher than anti-gp210 negative serum (χ2 = 11.17, P < 0. 01). Conclusions Multiple autoantibedies can be detected in the sera of PBC patients. The detection of autoantibody profile is useful for the diagnosis and differential diagnosis of PBC, and may he helpful for therapy and prognosis of PBC.
