CAJ | 학술논문

我们以前的研究建立了五株猕猴饲养层细胞系来支持猕猴胚胎干细胞(rESCs)的生长:一岁猴耳皮肤成纤维细胞(MESFs)、两岁猴输卵管成纤维细胞(MOFs)、成年猴卵泡颗粒成纤维样细胞(MFGs)、成年猴卵泡颗粒上皮样细胞(MFGEs),以及MESFs的克隆成纤维细胞(CMESFs).我们发现MESFs、CMESFs、MOFs和MFGs,而不足MFGEs支持猕猴胚胎干细胞(rESCs,rhesus embryonic stem cells)的生长.通过半定量PCR的方法,我们在支持性的饲养层细胞中检测到了一些基因的高表达.在本研究中,我们运用Affymetrix公司的GeneChip Rhesus Macaque Genome Array芯片来研究这五株同源饲养层的表达谱,希望发现哪些细胞因子和信号通路在维持rESCs中起到重要作用.结果表明,除MFGE外,包括GREM2、bFGF,、KITLG,、DKK3、GREM1、AREG、SERPINF1和LTBF1等八个基因的mRNA在支持性的饲养层细胞中高表达.本研究结果提示,很多信号通路在支持rESCs的未分化生长和多潜能性方面可能起到了冗余的作用.
아문이전적연구건립료오주미후사양층세포계래지지미후배태간세포(rESCs)적생장:일세후이피부성섬유세포(MESFs)、량세후수란관성섬유세포(MOFs)、성년후란포과립성섬유양세포(MFGs)、성년후란포과립상피양세포(MFGEs),이급MESFs적극륭성섬유세포(CMESFs).아문발현MESFs、CMESFs、MOFs화MFGs,이불족MFGEs지지미후배태간세포(rESCs,rhesus embryonic stem cells)적생장.통과반정량PCR적방법,아문재지지성적사양층세포중검측도료일사기인적고표체.재본연구중,아문운용Affymetrix공사적GeneChip Rhesus Macaque Genome Array심편래연구저오주동원사양층적표체보,희망발현나사세포인자화신호통로재유지rESCs중기도중요작용.결과표명,제MFGE외,포괄GREM2、bFGF,、KITLG,、DKK3、GREM1、AREG、SERPINF1화LTBF1등팔개기인적mRNA재지지성적사양층세포중고표체.본연구결과제시,흔다신호통로재지지rESCs적미분화생장화다잠능성방면가능기도료용여적작용.
In our previous study, five homologous feeder cell lines, Monkey ear skin fibroblasts (MESFs), clonally derived fibroblasts from the MESFs (CMESFs), monkey oviductal fibroblasts (MOFs), monkey follicular granulosa fibroblast-like (MFGs) cells, monkey follicular granulosa epithelium-like (MFGEs) cells, were developed for the maintenance of rhesus embryonic stem cells (rESCs). We found that MESFs, CMESFs, MOFs and MFGs, but not MFGEs, support the growth of rhesus embryonic stem cells. Moreover, we detected some genes that are upregulated in supportive feeder cell lines by semi-quantitative PCR. In the present study, we applied the GeneChip Rhesus Macaque Genome Array of Affymetrix Corporation to study the expression profiles of these five feeder cell lines, in purpose to find out which cytokines and signaling pathways were important in maintaining the rESCs, mRNAs of eight genes, including GREM2, bFGF, KITLG, DKK3, GREM1, AREG, SERPINF1 and LTBP1, were found to be upregulated in supportive feeder cell lines, but not in MFGE. The results indicate that many signaling pathways may play redundant roles in supporting the undifferentiated growth and maintenance of pluripotency in rESCs.