CAJ | 학술논문

目的建立测定杀鼠迷、杀鼠灵、溴敌隆和大隆4种杀鼠剂的高效液相色谱-电喷雾-质谱联用检测方法.方法采用ZORBAX sb-aq,150mm×2.1mm×3.5 μm(带预柱);柱温:30℃;流动相:甲酸(0.1%)甲醇和甲酸(0.1%)水溶液梯度条件;流速:0.4 mL/min;在负离子模式下,通过电喷雾电离(ESI),多反应离子临测(MRM)测定,外标法定量分析4种杀鼠剂,并测试方法的基质抑制作用.结果 4种鼠药在MRM筛选条件下,最小检出均在40pg以下;单个鼠药线性相关系数均在0.999以上,线性范围适当,最小检出限均在10pg以下.结论本实验方法可以作为检验生物检材中杀鼠迷、杀鼠灵、溴敌隆和大隆的筛选手段.
목적 건립측정살서미、살서령、추활륭화대륭4충살서제적고효액상색보-전분무-질보련용검측방법.방법 채용ZORBAX sb-aq,150mm×2.1mm×3.5 μm(대예주);주온:30℃;류동상:갑산(0.1%)갑순화갑산(0.1%)수용액제도조건;류속:0.4 mL/min;재부리자모식하,통과전분무전리(ESI),다반응리자림측(MRM)측정,외표법정량분석4충살서제,병측시방법적기질억제작용.결과 4충서약재MRM사선조건하,최소검출균재40pg이하;단개서약선성상관계수균재0.999이상,선성범위괄당,최소검출한균재10pg이하.결론 본실험방법가이작위검험생물검재중살서미、살서령、추활륭화대륭적사선수단.
Objective To set up a determination method of warfarin,coumatertralyl,bromadiolone and brodifacoum.Methods HPLC-ESI-MS/MS was used to detect warfarin,coumatertralyl,bromadiolone and brodifacoum using ZORBAX sb-aq(1 50mm×2.1 mm×3.5 μm)as chromatographic column at constant temperature 30℃ while methanol of 0.1%formic acid and water as mobile phase in the grads program.The flow rate was 0.4 mL/min;Detection was performed on an electrospray ionization(ESI)in the negative model and the MRM condition.Results The limit of qualitation was found to be less than 40 pg for four raticides at one time and 10pg for every raticide in the MRM condition;The four raticides linear range were suitable and r were more than 0.999.Matrix affection Was also determined.Conclusion This method is simple,sensitive and accurate for the determination of warfarin,coumatertralyl,bromadiolonc and brodifacoum in the biological tissue.