CAJ | 학술논문

在温室沙培灭菌条件下,以Al-P为磷源、枳为试材、Glomus mosseae(G. m)和G. versiforme(G. v)为菌剂,研究低磷胁迫下AM真菌对枳实生苗干物重、吸磷效应及根系分泌有机酸的影响.结果表明,接种AM真菌显著增加枳地上部、地下部干物重,增幅16.79%～135.25%;同时显著增加其吸磷量,菌丝对植株的吸磷贡献率为17.04%～71.95%(G.m>G.v),施Al-P显著提高菌丝吸磷贡献率.接种AM真菌的根系分泌的有机酸种类与对照有所不同,未接种处理枳分泌的有机酸有草酸、苹果酸、乳酸、乙酸、顺丁烯二酸和柠檬酸等6种,而接种G.m的则检测到草酸、酒石酸、苹果酸、乳酸、乙酸、柠檬酸、丁二酸等7种,G.v处理的检测到酒石酸,接种处理均未检测到顺丁烯二酸;接种丛枝菌根真菌增加了枳根系分泌有机酸的量(比未接种处理增加19.80～56.87 mg/kg,且施用AlPO_4后有机酸含量显著增加(增加20.06～21.84 mg/kg);未接种植株根系仅分泌少量有机酸;接种植株根系分泌的有机酸以苹果酸(42.87%)、柠檬酸(39.22%)和草酸(12.06%)为主.
재온실사배멸균조건하,이Al-P위린원、지위시재、Glomus mosseae(G. m)화G. versiforme(G. v)위균제,연구저린협박하AM진균대지실생묘간물중、흡린효응급근계분비유궤산적영향.결과표명,접충AM진균현저증가지지상부、지하부간물중,증폭16.79%～135.25%;동시현저증가기흡린량,균사대식주적흡린공헌솔위17.04%～71.95%(G.m>G.v),시Al-P현저제고균사흡린공헌솔.접충AM진균적근계분비적유궤산충류여대조유소불동,미접충처리지분비적유궤산유초산、평과산、유산、을산、순정희이산화저몽산등6충,이접충G.m적칙검측도초산、주석산、평과산、유산、을산、저몽산、정이산등7충,G.v처리적검측도주석산,접충처리균미검측도순정희이산;접충총지균근진균증가료지근계분비유궤산적량(비미접충처리증가19.80～56.87 mg/kg,차시용AlPO_4후유궤산함량현저증가(증가20.06～21.84 mg/kg);미접충식주근계부분비소량유궤산;접충식주근계분비적유궤산이평과산(42.87%)、저몽산(39.22%)화초산(12.06%)위주.
The effects of arbuscular mycorrhizal fungus (Glomus mosseae and G. versiforme) on the dry weight and absorbing phosphorus of Poncirus trifoliata seedlings and its root excreting organic acids under low-phosphorus stress with the Al-P as phosphorus source and poncirus trifoliata seedlings as experimental material were studied in sand culture and sterilization greenhouse. The results showed that the inoculation of AM not only markedly increased the dry weight of overground part and underground part of Poncirus trifoliata, which raised by 16.79%～135.25%, but also remarkably increased phosphorus uptake with the contribution rate up to 17.04%～71.95% (G.m>G.v). Fertilizing Al-p could observably raise the contribution rate. The kinds of organic acid which were secreted by the root systems of AM fungi inoculation were different from the control. We only detected six acids (oxalic acid, malic acid, lactic acid, acetic acid, maleic acid and citric acid) in non-inoculated treatment materials, while 7 acids (oxalic acid, tartaric acid, malic acid, lactic acid, acetic acid, citric acid, succinic acid) in G. m treatment materials, and also tartaric acid and no maleic acid detected in G. v treatment materials. Inoculation also increased the content of organic acid secretion (increased by 19.80～56.87 mg/kg) of trifoliate root, and the application of AlPO_4 greatly increased organic acid content (increased by 20.06～21.84 mg/kg); uninoculated plant root secreted a bit of organic acid; Mycorrhizal poncirus trifoliata seedlings excreted malic acid (42.87%), citric acid (39.22%) and oxalic acid (12.06%).