分子植物育种
分子植物育種
분자식물육충
MOLECULAR PLANT BREEDING
2010年
2期
335-339
,共5页
白菜型冬油菜%低温%抗寒性%生理生化指标
白菜型鼕油菜%低溫%抗寒性%生理生化指標
백채형동유채%저온%항한성%생리생화지표
Winter turnip rape (Brassica campetris)%Low temperature%Cold resistance%Biochemical and physiological indexes
为了了解渗透物质与油菜抗寒能力的关系,本研究以2个白菜型冬油菜陇油6号和天油4号为试验材料,通过冷冻处理,对油菜根部相对电导率和叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、可溶性蛋白、丙二醛(MDA)等一系列生理生化指标进行了检测.结果表明,陇油6号(DQW-1)的抗寒能力强于天油4号,陇油6号和天油4号的SOD和CAT活性、可溶性蛋白含量、MDA含量随着温度的降低均呈现出先上升后下降的变化规律.低温下耐寒性强的陇油6号能保持较高的SOD酶活性、CAT酶活性、可溶性蛋白和较低的MDA含量.因此,SOD和CAT活性、可溶性蛋白和MDA含量在一定程度上能反映白菜型冬油菜的抗寒性.
為瞭瞭解滲透物質與油菜抗寒能力的關繫,本研究以2箇白菜型鼕油菜隴油6號和天油4號為試驗材料,通過冷凍處理,對油菜根部相對電導率和葉片超氧化物歧化酶(SOD)、過氧化氫酶(CAT)、可溶性蛋白、丙二醛(MDA)等一繫列生理生化指標進行瞭檢測.結果錶明,隴油6號(DQW-1)的抗寒能力彊于天油4號,隴油6號和天油4號的SOD和CAT活性、可溶性蛋白含量、MDA含量隨著溫度的降低均呈現齣先上升後下降的變化規律.低溫下耐寒性彊的隴油6號能保持較高的SOD酶活性、CAT酶活性、可溶性蛋白和較低的MDA含量.因此,SOD和CAT活性、可溶性蛋白和MDA含量在一定程度上能反映白菜型鼕油菜的抗寒性.
위료료해삼투물질여유채항한능력적관계,본연구이2개백채형동유채롱유6호화천유4호위시험재료,통과냉동처리,대유채근부상대전도솔화협편초양화물기화매(SOD)、과양화경매(CAT)、가용성단백、병이철(MDA)등일계렬생리생화지표진행료검측.결과표명,롱유6호(DQW-1)적항한능력강우천유4호,롱유6호화천유4호적SOD화CAT활성、가용성단백함량、MDA함량수착온도적강저균정현출선상승후하강적변화규률.저온하내한성강적롱유6호능보지교고적SOD매활성、CAT매활성、가용성단백화교저적MDA함량.인차,SOD화CAT활성、가용성단백화MDA함량재일정정도상능반영백채형동유채적항한성.
In this paper,a series of biochemical and physiological indexes,such as electrolyte leakage,superoxide dimutase (SOD) activity,catalase (CAT) activity,soluble protein contents and malondialdehyde (MDA) contents,in the roots of two winter turnip rape (Brassica campetris) were examined in order to study the relationship between osmoticum and cold resistance of Brassica.The results showed that cold resistance of DQW-1 was stronger than that of Tianyou No.4,The SOD and CAT activities,soluble protein content and MDA content presented up-down trend with the decrease of temperature.DQW-1 with stronger cold resistance under low temperature showed higher SOD and CAT activities,higher solutble protein content,and lower MDA content.Therefore,the activities of SOD and CAT,soluble protein content and MDA content maybe reflect the cold resistant of winter turnip rape in some degree.
