中成药
中成藥
중성약
CHINESE TRADITIONAL PATENT MEDICINE
2010年
12期
2191-2194
,共4页
HPLC%抗风湿类中成药%非甾体类化学物质
HPLC%抗風濕類中成藥%非甾體類化學物質
HPLC%항풍습류중성약%비치체류화학물질
目的:建立HPLC法检测抗风湿类中成药中非法添加非甾体类化学物质萘普生、芬布芬、保泰松、双氯芬酸钠、吲哚美辛及布洛芬的检验方法.方法:采用Diamonsii C18色谱柱,流动相:甲醇-0.01 mol/L磷酸二氢钾溶液(用磷酸调pH至3.3)(66:34),流速:1.0 mL/min,柱温:20℃,检测波长:263 nm.结果:萘普生对照品线性范围34.08～68.16g/mL,r=0.9991;芬布芬对照品线性范围29.64～59.28 ug/mL,r=0.9992;保泰松对照品线性范围203.48～406.96μg/mL,r=0.9997;双氯芬酸钠对照品线性范围84.66～169.32μg/mL,r=0.9999;吲哚美辛对照品线性范围79.50～159.00 μg/mL,r=0.9999;布洛芬对照品线性范闱206.60～413.20μg/mL,r=0.9997.平均回收率分别为99.34%,98.39%,98.61%,97.49%,99.28%,98.18%,RSD分别为1.1%,1.4%,0.8%,0.8%,1.5%,0.7%(n=6).结论:本方法灵敏度高,操作简便、准确,可用于定性和定量检测抗风湿类中成药中非法添加的非甾体类化学物质萘普生、芬布芬、保泰松、双氯芬酸钠、吲哚美辛及布洛芬.
目的:建立HPLC法檢測抗風濕類中成藥中非法添加非甾體類化學物質萘普生、芬佈芬、保泰鬆、雙氯芬痠鈉、吲哚美辛及佈洛芬的檢驗方法.方法:採用Diamonsii C18色譜柱,流動相:甲醇-0.01 mol/L燐痠二氫鉀溶液(用燐痠調pH至3.3)(66:34),流速:1.0 mL/min,柱溫:20℃,檢測波長:263 nm.結果:萘普生對照品線性範圍34.08～68.16g/mL,r=0.9991;芬佈芬對照品線性範圍29.64～59.28 ug/mL,r=0.9992;保泰鬆對照品線性範圍203.48～406.96μg/mL,r=0.9997;雙氯芬痠鈉對照品線性範圍84.66～169.32μg/mL,r=0.9999;吲哚美辛對照品線性範圍79.50～159.00 μg/mL,r=0.9999;佈洛芬對照品線性範闈206.60～413.20μg/mL,r=0.9997.平均迴收率分彆為99.34%,98.39%,98.61%,97.49%,99.28%,98.18%,RSD分彆為1.1%,1.4%,0.8%,0.8%,1.5%,0.7%(n=6).結論:本方法靈敏度高,操作簡便、準確,可用于定性和定量檢測抗風濕類中成藥中非法添加的非甾體類化學物質萘普生、芬佈芬、保泰鬆、雙氯芬痠鈉、吲哚美辛及佈洛芬.
목적:건립HPLC법검측항풍습류중성약중비법첨가비치체류화학물질내보생、분포분、보태송、쌍록분산납、신타미신급포락분적검험방법.방법:채용Diamonsii C18색보주,류동상:갑순-0.01 mol/L린산이경갑용액(용린산조pH지3.3)(66:34),류속:1.0 mL/min,주온:20℃,검측파장:263 nm.결과:내보생대조품선성범위34.08～68.16g/mL,r=0.9991;분포분대조품선성범위29.64～59.28 ug/mL,r=0.9992;보태송대조품선성범위203.48～406.96μg/mL,r=0.9997;쌍록분산납대조품선성범위84.66～169.32μg/mL,r=0.9999;신타미신대조품선성범위79.50～159.00 μg/mL,r=0.9999;포락분대조품선성범위206.60～413.20μg/mL,r=0.9997.평균회수솔분별위99.34%,98.39%,98.61%,97.49%,99.28%,98.18%,RSD분별위1.1%,1.4%,0.8%,0.8%,1.5%,0.7%(n=6).결론:본방법령민도고,조작간편、준학,가용우정성화정량검측항풍습류중성약중비법첨가적비치체류화학물질내보생、분포분、보태송、쌍록분산납、신타미신급포락분.
