中华耳鼻咽喉头颈外科杂志
中華耳鼻嚥喉頭頸外科雜誌
중화이비인후두경외과잡지
CHINESE JOURNAL OF OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2008年
7期
524-528
,共5页
血管内皮生长因子C%鼻咽肿瘤%淋巴管生成
血管內皮生長因子C%鼻嚥腫瘤%淋巴管生成
혈관내피생장인자C%비인종류%림파관생성
Vascular endothelial growth factor C%Nasopharyngeal neoplasms%Lymphangiogenesis
目的 检测鼻咽癌中血管内皮生成因子C(VEGF-C)的表达及微淋巴管密度(microlymphatic vessel density,MLVD),探讨鼻咽癌淋巴管生成及淋巴转移之间的关系.方法 采用免疫组化SP法检测58例鼻咽癌和20例鼻咽部炎性反应组织中VEGF-C的表达情况,并用淋巴管内皮细胞特异性抗体LYVE-1行免疫组化染色,计数肿瘤内MLVD,并结合临床病理特征进行分析.结果 鼻咽癌组和炎性反应对照组VEGF-C的阳性表达率分别为84.5%(49/58)和15.0%(3/20),差异有统计学意义(X2=32.309,P＜0.01).鼻咽癌组织MLVD为(28.6±6.2)个/视野,鼻咽炎性反应组为(10.5 4±3.0)个/视野,两组差异有统计学意义(t=12.491,P＜0.01).鼻咽癌组织中,有淋巴转移组VEGF-C阳性表达率(87.8%)明显高于无淋巴转移组(76.5%);有淋巴转移组MLVD(30.2 4±6.4)个/视野高于无淋巴转移组(24.8±3.6)个/视野,经统计学分析,差异均有统计学意义(t=3.259,P＜0.01).VEGF-C的表达与MLVD(t=3.512,P＜0.01)、淋巴转移(X2=7.715,P＜0.01)、临床分期(X2=4.250,P＜0.05),与病理分化程度无关(X2=0.000,P＞0.05).VEGF-C的表达与MLVD(t=3.512,P＜0.01)、淋巴转移(X2=7.715,P＜0.01,r=0.712)、临床分期(X2=4.250,P＜0.05,r=0.481)相关,与病理分化程度无关(X2=0.000,P＞0.05).结论 在鼻咽癌组织中VEGF-C呈高表达,VEGF-C表达与鼻咽癌组织MLVD、淋巴转移、临床分期密切相关.VEGF-C可能参与了鼻咽癌发生、浸润和转移的过程.VEGF-C与肿瘤组织微淋巴管密切相关,在鼻咽癌发展过程中起重要作用,可能成为抗肿瘤治疗的潜在靶点.
目的 檢測鼻嚥癌中血管內皮生成因子C(VEGF-C)的錶達及微淋巴管密度(microlymphatic vessel density,MLVD),探討鼻嚥癌淋巴管生成及淋巴轉移之間的關繫.方法 採用免疫組化SP法檢測58例鼻嚥癌和20例鼻嚥部炎性反應組織中VEGF-C的錶達情況,併用淋巴管內皮細胞特異性抗體LYVE-1行免疫組化染色,計數腫瘤內MLVD,併結閤臨床病理特徵進行分析.結果 鼻嚥癌組和炎性反應對照組VEGF-C的暘性錶達率分彆為84.5%(49/58)和15.0%(3/20),差異有統計學意義(X2=32.309,P＜0.01).鼻嚥癌組織MLVD為(28.6±6.2)箇/視野,鼻嚥炎性反應組為(10.5 4±3.0)箇/視野,兩組差異有統計學意義(t=12.491,P＜0.01).鼻嚥癌組織中,有淋巴轉移組VEGF-C暘性錶達率(87.8%)明顯高于無淋巴轉移組(76.5%);有淋巴轉移組MLVD(30.2 4±6.4)箇/視野高于無淋巴轉移組(24.8±3.6)箇/視野,經統計學分析,差異均有統計學意義(t=3.259,P＜0.01).VEGF-C的錶達與MLVD(t=3.512,P＜0.01)、淋巴轉移(X2=7.715,P＜0.01)、臨床分期(X2=4.250,P＜0.05),與病理分化程度無關(X2=0.000,P＞0.05).VEGF-C的錶達與MLVD(t=3.512,P＜0.01)、淋巴轉移(X2=7.715,P＜0.01,r=0.712)、臨床分期(X2=4.250,P＜0.05,r=0.481)相關,與病理分化程度無關(X2=0.000,P＞0.05).結論 在鼻嚥癌組織中VEGF-C呈高錶達,VEGF-C錶達與鼻嚥癌組織MLVD、淋巴轉移、臨床分期密切相關.VEGF-C可能參與瞭鼻嚥癌髮生、浸潤和轉移的過程.VEGF-C與腫瘤組織微淋巴管密切相關,在鼻嚥癌髮展過程中起重要作用,可能成為抗腫瘤治療的潛在靶點.
목적 검측비인암중혈관내피생성인자C(VEGF-C)적표체급미림파관밀도(microlymphatic vessel density,MLVD),탐토비인암림파관생성급림파전이지간적관계.방법 채용면역조화SP법검측58례비인암화20례비인부염성반응조직중VEGF-C적표체정황,병용림파관내피세포특이성항체LYVE-1행면역조화염색,계수종류내MLVD,병결합림상병리특정진행분석.결과 비인암조화염성반응대조조VEGF-C적양성표체솔분별위84.5%(49/58)화15.0%(3/20),차이유통계학의의(X2=32.309,P＜0.01).비인암조직MLVD위(28.6±6.2)개/시야,비인염성반응조위(10.5 4±3.0)개/시야,량조차이유통계학의의(t=12.491,P＜0.01).비인암조직중,유림파전이조VEGF-C양성표체솔(87.8%)명현고우무림파전이조(76.5%);유림파전이조MLVD(30.2 4±6.4)개/시야고우무림파전이조(24.8±3.6)개/시야,경통계학분석,차이균유통계학의의(t=3.259,P＜0.01).VEGF-C적표체여MLVD(t=3.512,P＜0.01)、림파전이(X2=7.715,P＜0.01)、림상분기(X2=4.250,P＜0.05),여병리분화정도무관(X2=0.000,P＞0.05).VEGF-C적표체여MLVD(t=3.512,P＜0.01)、림파전이(X2=7.715,P＜0.01,r=0.712)、림상분기(X2=4.250,P＜0.05,r=0.481)상관,여병리분화정도무관(X2=0.000,P＞0.05).결론 재비인암조직중VEGF-C정고표체,VEGF-C표체여비인암조직MLVD、림파전이、림상분기밀절상관.VEGF-C가능삼여료비인암발생、침윤화전이적과정.VEGF-C여종류조직미림파관밀절상관,재비인암발전과정중기중요작용,가능성위항종류치료적잠재파점.
Objective To detect the expression of vessel endothelial growth factorc(VEGF-C)and microlymphatic vessel density(MLVD)and probe the relationship between lymphangiogenesis and lymph node micrometastasis in nasopharyngeal carcinoma.Methods The expression of VEGF-C,was evaluated in 58 cases with nasopharyngeal carcinoma and 20 samples of nasopharyngitis tissues by immunohistochemical staining SP methods.The MLVD in tumor was counted by immunostaining with the specific antibody lymphatic vessel endothelial hyaluronic acid receptor 1 and analyzed with clinieopathologic parameters.Results The positive rate of VEGF-C was 84.5% and 15.0% respectively in nasopharyngeal carcinoma and nasopharyngitis tissues,there was significant difference between them(X2=32.309,P＜0.01).The microlymphatic vessel density was(28.6±6.2)pieces per field and(10.5±3.0)pieces per field respectively in nasopharyngeal carcinoma and nasopharyngitis tissues,there was significant difference between them(t=12.491,P＜0.01).The expression of VEGF-C and the MLVD were significantly higher in nasopharyngeal carcinoma and in lymph node mesastasis group than in no-metastasis group.The expression of VEGF-C was positively correlated with MLVD(t=3.512,P＜0.01),lymph node metastasis(X2=7.715,P＜0.01,r=0.712)and clinical stage(X2=4.250,P＜0.05,r=0.481).Conclusions In nasopharyngeal carcinoma,the expression rate of VEGF-C was high.VEGF-C expression was positively correlated with MLVD and lymph node metastasis and tumor staging.It was explained that VEGF-C attached itself to the emergence and infihration and transfer of NPC.There was close correlation between the expression of VEGF-C and MLVD.It was explained that the expression of VEGF-C in NPC was correlated to neoplasm lymphangiogenesis and that VEGF-C played a vital role in the progression of NPC.VEGF-C Was likely to a potential target of antieareer therapy.
