CAJ | 학술논문

目的探讨神经生长因子受体p75(p75NGFR)在膀胱癌组织中的表达及缺氧条件下在膀胱癌细胞表达的变化.方法免疫组织化学方法检测107例膀胱癌组织标本(男85例,女22例,平均年龄61岁;T117例、T2 77例、T3～T4 13例;病理分级Ⅰ～Ⅱ级75例、Ⅲ～Ⅳ级32例)和24例转移淋巴结组织标本中p75NGFR的表达,癌旁正常黏膜组织50例为对照.选取膀胱移行细胞癌T24细胞株,免疫组织化学、半定量RT-PCR方法检测常规氧分压和微缺氧条件下 p75NGFR在细胞株中的表达.结果 107例膀胱癌组织标本中p75NGFR阳性表达46例(43.0%),正常对照组标本均为阴性表达.p75NGFR阳性表达率与患者性别、年龄、肿瘤组织学分级和分期无相关性(x2值分别为1.509、3.759、0.011和2.324,P值均0.05).24例转移淋巴结组织标本中p75NGFR阳性表达5例(20.8%),与膀胱癌组织标本阳性率比较差异有统计学意义(r=0.077,P<0.05).微缺氧条件下第3天T24细胞株的p75NGFR mRNA吸光度值为0.52±0.07,正常对照组为0.91±0.01,组间差异有统计学意义(P<0.05).结论膀胱癌组织中p75NGFR表达与淋巴结转移呈负相关;微缺氧条件下p75NGFR mRNA在膀胱癌细胞株中的表达降低.
목적 탐토신경생장인자수체p75(p75NGFR)재방광암조직중적표체급결양조건하재방광암세포표체적변화.방법 면역조직화학방법검측107례방광암조직표본(남85례,녀22례,평균년령61세;T117례、T2 77례、T3～T4 13례;병리분급Ⅰ～Ⅱ급75례、Ⅲ～Ⅳ급32례)화24례전이림파결조직표본중p75NGFR적표체,암방정상점막조직50례위대조.선취방광이행세포암T24세포주,면역조직화학、반정량RT-PCR방법검측상규양분압화미결양조건하 p75NGFR재세포주중적표체.결과 107례방광암조직표본중p75NGFR양성표체46례(43.0%),정상대조조표본균위음성표체.p75NGFR양성표체솔여환자성별、년령、종류조직학분급화분기무상관성(x2치분별위1.509、3.759、0.011화2.324,P치균0.05).24례전이림파결조직표본중p75NGFR양성표체5례(20.8%),여방광암조직표본양성솔비교차이유통계학의의(r=0.077,P<0.05).미결양조건하제3천T24세포주적p75NGFR mRNA흡광도치위0.52±0.07,정상대조조위0.91±0.01,조간차이유통계학의의(P<0.05).결론 방광암조직중p75NGFR표체여림파결전이정부상관;미결양조건하p75NGFR mRNA재방광암세포주중적표체강저.
Objective To explore the expression of the nerve growth factor receptors p75 (p75NGFR) in human bladder carcinoma samples, and the effects of hypoxia on the expression of p75NGFR in human bladder cancer ceils. Methods The expression of p75NGFR in 107 bladder cancer and lymph node specimens were immunohistochemically investigated. The expression of p75NGFR in bladder cancer cell line (T24) was assessed by immunocytochemistry, and reverse tran-scription-PCR in the Normoxic Condition (air, 5%CO2) and in hypoxia condition(10%O2, 5%CO2, 85%N2). Results p75NGFR expressed in 46 of 107(43.0%) tumor samples. There was no signifi-cant correlation between p75NGFR and the factors such as gender, age, extent of the tumors, and pathologic grading(P0.05), p75NGFR was expressed in examined cell line T24, and also expressed in 5 of 24 bladder tumors in metastasized lymph node specimens. Hypoxia markedly down-regulated the expression of p75NGFR of T24 cell line at third day. Conclusions It is suggested that p75NGFR is expressed less in lymph node metastasis. Hypoxia markedly inhibited expression of p75NGFR of T24 cell lines.