CAJ | 학술논문

目的应用RNA干扰技术下调小鼠肝癌细胞株(Hca-F)COL8A1的表达,通过观察癌细胞的体外增殖、侵袭能力,研究调节COL8A1表达对肿瘤细胞药敏性的影响.方法采用RTPCR、Western Blot分析干扰前后Hca-F细胞中COL8A1的表达情况.用体外侵袭实验检测干扰前后Hca-F细胞的侵袭能力.用MTT法分析干扰前后Hca-F细胞的增殖情况及其对右旋柠烯(D-limonene)的药敏性.结果 Hca-F/RNAi细胞中COL8A1的表达与未给予RNA干扰处理的对照组及RNA干扰对照组相比出现明显下调;该细胞的增殖及穿过基质胶的侵袭能力下降;同时上调该细胞对D-limonene的药敏性.结论小鼠肝癌细胞中COL8A1的表达与肿瘤细胞的增殖、侵袭及对抗肿瘤药物的敏感性密切相关,可能为肿瘤的化学治疗提供新靶点.
목적 응용RNA간우기술하조소서간암세포주(Hca-F)COL8A1적표체,통과관찰암세포적체외증식、침습능력,연구조절COL8A1표체대종류세포약민성적영향.방법 채용RTPCR、Western Blot분석간우전후Hca-F세포중COL8A1적표체정황.용체외침습실험검측간우전후Hca-F세포적침습능력.용MTT법분석간우전후Hca-F세포적증식정황급기대우선저희(D-limonene)적약민성.결과 Hca-F/RNAi세포중COL8A1적표체여미급여RNA간우처리적대조조급RNA간우대조조상비출현명현하조;해세포적증식급천과기질효적침습능력하강;동시상조해세포대D-limonene적약민성.결론 소서간암세포중COL8A1적표체여종류세포적증식、침습급대항종류약물적민감성밀절상관,가능위종류적화학치료제공신파점.
Objective To investigate the possible effects of COL8A1 on the proliferation, invasion and drug sensitivity of murine hepatocarcinoma cell line Hca-F, we used an RNA interference (RNAi) approach to silence COL8A1 expression. Methods The expression levels of COL8A1 in HcaF/siRNA cells were assessed by RT-PCR and Western blot. The inhibitory effect of RNAi on Hca-F cell invasion in vitro was demonstrated by ECM invasion assay. The in vitro proliferative ability and drug sensitivity of COL8A1-deficient cells were determined by MTT assay. Results The expression of COL8A1 was significantly reduced in COL8A1/siRNA cells after 30h transfection, compared with both the RNAi control and the Hca-F cells. The reduced COL8A1 expression also attenuated the proliferative, invasive ability, as well as increased drug sensitivity of Hca-F/siRNA cells. Conclusion Our current results indicate that the expression of COL8A1 functionally mediates tumor cell proliferation, invasion, and drug sensitivity, and is a potential target for therapeutic anti-cancer drugs.