CAJ | 학술논문

目的在表达绿色荧光蛋白( GFP)裸小鼠脑内建立人脑多形性胶质母细胞瘤(GBM)原位移植模型.方法将表达GFP的C57/B6鼠与BALB/c nu/nu系裸小鼠杂交筛选出表达GFP的裸小鼠.把人脑GBM组织原位移植于GFP裸小鼠脑内,荧光显微镜下观察移植瘤的生长特性、血管生成及表皮生长因子受体(EGFR)表达.结果成功培育出表达GFP的荧光裸小鼠；移植瘤中EGFR阳性表达率(78.6±5.2)％,肿瘤侵袭性生长并血管丰富.结论人脑GBM荧光裸小鼠原位移植模型能模拟人脑原发恶性胶质瘤高侵袭及EGFR高表达的生物学特性.
목적 재표체록색형광단백( GFP)라소서뇌내건립인뇌다형성효질모세포류(GBM)원위이식모형.방법 장표체GFP적C57/B6서여BALB/c nu/nu계라소서잡교사선출표체GFP적라소서.파인뇌GBM조직원위이식우GFP라소서뇌내,형광현미경하관찰이식류적생장특성、혈관생성급표피생장인자수체(EGFR)표체.결과 성공배육출표체GFP적형광라소서；이식류중EGFR양성표체솔(78.6±5.2)％,종류침습성생장병혈관봉부.결론 인뇌GBM형광라소서원위이식모형능모의인뇌원발악성효질류고침습급EGFR고표체적생물학특성.
Objective To establish orthotopic xenograft model of human glioblastoma multiforme (GBM) in the brain of nude mice with green fluorescent protein (GFP) expression.Methods Nude mice with ubiquitous GFP expression were obtained by crossing BALB/c nu/nu nude mice with transgenic C57/B6-GFP mice.Then,the fresh tumor tissues of human GBM were orthotopically implanted into the brains of GFP nude mice.The biologic features with growth pattern,angiogenesis and epidermal growth factor receptor (EGFR) expression were determined by fluorescence microscopy.Results GFP nude mice were generated by reciprocally crossing female C57/B6-GFP mice with male BALB/c nu/nu nude mice.The orthotopic xenografts showed invasive behavior,high EGFR expression profile with (78.6 ± 5.2 )％ positive rate,and rich angiogenesis under fluorescence background.Conclusion The orthotopic xenografts from human GBM grown in the GFP nude mice shared the same biological features with high EGFR expression and invasiveness with its counterpart in the original tumors.