CAJ | 학술논문

目的探讨SV40Tag、Rb和IRS-1在胶质瘤发生发展过程中的关系和作用.方法构建布有人脑胶质瘤和脑膜瘤临床标本为主体、配有实验性胶质瘤、正常人或鼠脑等相关组织等118个阵列的组织芯片,采用免疫组织化学和免疫荧光共聚焦技术检测SV40Tag、Rb和IRS-1的表达及SV40Tag与Rb、SV40Tag与IRS-1的联合表达.结果 SV40Tag、Rb和IRS-1在胶质瘤和脑膜瘤中均有表达,82例胶质瘤的阳性表达率分别为65.9%、64.6%和48.8%.正常人脑组织仅有Rb和IRS-1表达,而未见SV40Tag表达.SV40Tag和IRS-1的阳性表达率与病理分级呈正相关(P<0.05),Rb蛋白的阳性表达率与病理分级呈负相关(P<0.05).在82例胶质瘤中,SV40Tag与Rb、SV40Tag与IRS-1联合表达阳性率分别为51.2%和40.2%.结论外源性SV40Tag随SV40病毒侵入机体,与Rb抑癌基因形成复合物使其抑癌功能丧失,同时诱导信号通路中重要成员IRS-1活化,从而促进细胞恶性变化,可能是脑胶质瘤发生发展的重要原因之一.
목적 탐토SV40Tag、Rb화IRS-1재효질류발생발전과정중적관계화작용.방법 구건포유인뇌효질류화뇌막류림상표본위주체、배유실험성효질류、정상인혹서뇌등상관조직등118개진렬적조직심편,채용면역조직화학화면역형광공취초기술검측SV40Tag、Rb화IRS-1적표체급SV40Tag여Rb、SV40Tag여IRS-1적연합표체.결과 SV40Tag、Rb화IRS-1재효질류화뇌막류중균유표체,82례효질류적양성표체솔분별위65.9%、64.6%화48.8%.정상인뇌조직부유Rb화IRS-1표체,이미견SV40Tag표체.SV40Tag화IRS-1적양성표체솔여병리분급정정상관(P<0.05),Rb단백적양성표체솔여병리분급정부상관(P<0.05).재82례효질류중,SV40Tag여Rb、SV40Tag여IRS-1연합표체양성솔분별위51.2%화40.2%.결론 외원성SV40Tag수SV40병독침입궤체,여Rb억암기인형성복합물사기억암공능상실,동시유도신호통로중중요성원IRS-1활화,종이촉진세포악성변화,가능시뇌효질류발생발전적중요원인지일.
Objective To determine the expression of SV40Tag, Rb and IRS-1 in gliomas and to identify their function in gliomagenesis and progression. Methods Tissue microarrays were constructed containing 118 samples including human glioma and meningioma, experimental glioma, and normal human brain tissue. The expression of SV40Tag, Rb, IRS-1, SV40Tag combined with Rb, and SV40Tag combined with IRS-1 were assayed by immunofluorescence or immunohistochemical techniques. The expression ratio and level were analyzed. Results The expressions of SV40Tag, Rb and IRS-1 were detected in gliomas and benign brain tumors. Their positive expression rate in glioma was 65.9%, 64.6% and 48.8%, respectively, with a statistically non-significant difference between the malignant and benign brain tumors. The malignant degree was positively correlated with SV4OTag and IRS-1, but negatively correlated with Rb expression. The combined expression rate of SV40Tag and Rb was 51.2%, and the combined expression rate of SV40Tagand IRS-1 was 40.2%. In the normal human brain tissue only the expression of Rb (77.8%, 7/9) andIRS-1 (22.2%, 2/9) were detected, but expression of SV40Tag could not be observed. Conclusion Our findings that no expression of SV40Tag was observed in normal human brain tissue indicates that expression of SV40Tag may play an important role in the pathogenesis of glioma. Itr may be assumed that after SV40virus invading human body, Rb disfunction and IRS-1 activation promote the malignant transformation of cells, which could be one of important factors in pathogenesis and procession of glioms.