CAJ | 학술논문

目的探讨IFN-γ和IL-6对多发性骨髓瘤(multiple myeloma,MM)B淋巴细胞刺激因子(BLyS)表达变化的影响及相关机制.方法采用流式细胞术、实时荧光定量PCR、E1.ISA及Western blot方法分析人MM肿瘤细胞KM3在IFN-γ和IL-6以及相关信号通路特异性抑制剂作用前后BLJys表达水平的变化.结果 lFN-γ和IL-6促进KM3细胞BLyS的表达水平;NF-KB抑制剂能够抑制BLyS的表达水平;NF-KB抑制剂BAY11-7082能够完全下调IFN-γ引起的BLyS表达的上调作用;抑制促分裂原活化蛋白激酶(MAPK)的活性能够下调BLyS的表达水平.结论 MAPK与NF-KB信号通路参与了Blys的表达调节.
목적 탐토IFN-γ화IL-6대다발성골수류(multiple myeloma,MM)B림파세포자격인자(BLyS)표체변화적영향급상관궤제.방법 채용류식세포술、실시형광정량PCR、E1.ISA급Western blot방법 분석인MM종류세포KM3재IFN-γ화IL-6이급상관신호통로특이성억제제작용전후BLJys표체수평적변화.결과 lFN-γ화IL-6촉진KM3세포BLyS적표체수평;NF-KB억제제능구억제BLyS적표체수평;NF-KB억제제BAY11-7082능구완전하조IFN-γ인기적BLyS표체적상조작용;억제촉분렬원활화단백격매(MAPK)적활성능구하조BLyS적표체수평.결론 MAPK여NF-KB신호통로삼여료Blys적표체조절.
Objective To investigate the regulation of B-lymphocyte stimulator(BLyS) levels in response to IFN-γand IL-6. Methods Flow cytometry, quantitative polymerase chain reaction, ELISA and Western blot were applied to examine the expression level of BLyS in response to IFN-γ and IL-6 . Results IFN-γand IL-6 induced BLyS expression in KM3 cells. After treated with BAY11-7082, an IkB-α phospho- rylation inhibitor, the up regulation of BI,yS induced by IFN-γ was completely inhibited. Inhibiting the nu-clear faetor-kB (NF-kB) and mitogen activated protein kinase(MAPK) activation in KM3 cells reduced BLyS protein and gene expression. Conclusion MAPK and NF-kB pathways are involved in the regulation of BLyS expression, which suggests that MAPK and NF-kB might be used for the treatment of multiple mye- loma.