CAJ | 학술논문

目的研究电针关元穴对脊髓损伤后尿潴留大鼠逼尿肌兴奋性和脑源性神经营养因子(BD.NF)与酪氨酸激酶受体B(TrkB)在的脊髓中表达水平的影响及作用机制.方法按照重物坠击方法建立脊髓损伤后尿潴留模型,将80只SD雌性大鼠随机分为正常组、假手术组、模型对照组和电针关元穴治疗组(电针组),每组20只,电针组每天给予电针治疗.共治疗10 d,其它组无治疗,10 d后采用免疫组织化学染色法对4组大鼠T6～T10椎体脊髓中BDNF及其受体TrkB的表达水平进行检测,并通过肌条实验测定各组大鼠离体逼尿肌兴奋性.结果结果显示,电针组和模型对照组脊髓中BDNF及其受体TrkB的表达水平以及逼尿肌兴奋性与正常组和假手术组比较差异均有统计学意义(P<0.05),且电针组与模型对照组比较,差异也有统计学意义(P<0.05).结论电针关元穴可促进脊髓中具有营养和修复神经功能的BDNF及TrkB的表达水平的提高,使受损的具有传导排尿反射冲动功能的中枢神经及其神经通路得到修复,从而增强逼尿肌排尿反射活动及其兴奋性.
목적 연구전침관원혈대척수손상후뇨저류대서핍뇨기흥강성화뇌원성신경영양인자(BD.NF)여락안산격매수체B(TrkB)재적척수중표체수평적영향급작용궤제.방법 안조중물추격방법건립척수손상후뇨저류모형,장80지SD자성대서수궤분위정상조、가수술조、모형대조조화전침관원혈치료조(전침조),매조20지,전침조매천급여전침치료.공치료10 d,기타조무치료,10 d후채용면역조직화학염색법대4조대서T6～T10추체척수중BDNF급기수체TrkB적표체수평진행검측,병통과기조실험측정각조대서리체핍뇨기흥강성.결과 결과현시,전침조화모형대조조척수중BDNF급기수체TrkB적표체수평이급핍뇨기흥강성여정상조화가수술조비교차이균유통계학의의(P<0.05),차전침조여모형대조조비교,차이야유통계학의의(P<0.05).결론 전침관원혈가촉진척수중구유영양화수복신경공능적BDNF급TrkB적표체수평적제고,사수손적구유전도배뇨반사충동공능적중추신경급기신경통로득도수복,종이증강핍뇨기배뇨반사활동급기흥강성.
Objective To study the effect and mechanism of electroaeupuncture at acupoint Guanyuan on expression of brain-derived neurotrophic factor (BDNF) and tropomyosine receptor kinase B (TrkB) in spinal cord and excitability of detrusor of rat with urinary retention after spinal cord injury. Methods The model of urinary retention after spinal cord injury was established by using weight drop method. Eighty female Sprague-Dawley rats were randomly divided into four groups, namely, a normal, a sham, a model and an electroacupuncture (EA) group, with 20 rats in each group. The EA group was given electroacupuncture at aeupoint Guanyuau once a day for 10 days in total, while the other groups was given no treatment. After 10 days, the expression level of BDNF and TrkB in spinal cord of injury site was measured by immunohistochemistry staining, and excitability of detrusor was measured by using the muscle strips in vitro. Results It was revealed that, with regard to the expression level of BDNF and TrkB in spinal cord,the model group exceeded the normal and sham group, the EA group significantly exceeded the other groups ( P < 0. 05 ). With regard to the excitability of detrusor, the model group was significant-ly lower than the normal and sham group. The EA group significantly exceeded the model group ( P < 0.05 ). Conclusion Electroacupuncture at acupoint Guanyuan can raise the expression level of BDNF and TrkB in spinal cord and increase the excitability of detrusor in rats with urinary retention after spinal cord injury.