中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2012年
1期
48-51
,共4页
射频%非损伤嫩肤%胶原蛋白%胶原纤维%成纤维细胞
射頻%非損傷嫩膚%膠原蛋白%膠原纖維%成纖維細胞
사빈%비손상눈부%효원단백%효원섬유%성섬유세포
Viemedic (RF)%Non-ablative photorejuvnation%Collagen%Collagen fibers%Fihrohlast
目的 评估使用唯美德科射频美容按摩仪(Viemedie RF AS,C0 11C)非损伤嫩肤治疗昆明小鼠后真皮胶原蛋白含量的变化,为临床嫩肤治疗提供理论依据.方法 Viemedic射频仪对20只昆明小鼠进行非损伤嫩肤治疗,每只小鼠背部皮肤以脊柱为分界线分为左右两侧,右侧为治疗侧,接受射频治疗；左侧为对照侧,不接受射频治疗.共治疗5次,每次间隔7d,每次治疗10 min.治疗结束后,免疫组织化学检测20只昆明小鼠真皮中胶原蛋白的含量；HE染色测定真皮胶原纤维的厚度及分布；胶原纤维染色,对比治疗侧和对照侧胶原纤维的面积密度.结果 组织学显示治疗侧表皮变平,胶原厚度增加,成纤维细胞及真皮胶原纤维增多、密集分布.胶原纤维图像定量分析显示,治疗后真皮中胶原纤维的面积密度均值明显高于治疗前的均值,差异有统计学意义(P＜0.01).结论 射频非损伤嫩肤治疗昆明小鼠,是安全有效的嫩肤方法.
目的 評估使用唯美德科射頻美容按摩儀(Viemedie RF AS,C0 11C)非損傷嫩膚治療昆明小鼠後真皮膠原蛋白含量的變化,為臨床嫩膚治療提供理論依據.方法 Viemedic射頻儀對20隻昆明小鼠進行非損傷嫩膚治療,每隻小鼠揹部皮膚以脊柱為分界線分為左右兩側,右側為治療側,接受射頻治療；左側為對照側,不接受射頻治療.共治療5次,每次間隔7d,每次治療10 min.治療結束後,免疫組織化學檢測20隻昆明小鼠真皮中膠原蛋白的含量；HE染色測定真皮膠原纖維的厚度及分佈；膠原纖維染色,對比治療側和對照側膠原纖維的麵積密度.結果 組織學顯示治療側錶皮變平,膠原厚度增加,成纖維細胞及真皮膠原纖維增多、密集分佈.膠原纖維圖像定量分析顯示,治療後真皮中膠原纖維的麵積密度均值明顯高于治療前的均值,差異有統計學意義(P＜0.01).結論 射頻非損傷嫩膚治療昆明小鼠,是安全有效的嫩膚方法.
목적 평고사용유미덕과사빈미용안마의(Viemedie RF AS,C0 11C)비손상눈부치료곤명소서후진피효원단백함량적변화,위림상눈부치료제공이론의거.방법 Viemedic사빈의대20지곤명소서진행비손상눈부치료,매지소서배부피부이척주위분계선분위좌우량측,우측위치료측,접수사빈치료；좌측위대조측,불접수사빈치료.공치료5차,매차간격7d,매차치료10 min.치료결속후,면역조직화학검측20지곤명소서진피중효원단백적함량；HE염색측정진피효원섬유적후도급분포；효원섬유염색,대비치료측화대조측효원섬유적면적밀도.결과 조직학현시치료측표피변평,효원후도증가,성섬유세포급진피효원섬유증다、밀집분포.효원섬유도상정량분석현시,치료후진피중효원섬유적면적밀도균치명현고우치료전적균치,차이유통계학의의(P＜0.01).결론 사빈비손상눈부치료곤명소서,시안전유효적눈부방법.
Objective To evaluate the change of the amount of collagen in the viemedic RF ASC01IC non-ablative photorejuveantion,in order to provide reference for effective clinical treatment.Methods Twenty rats were randomly divided into two groups,with ten rats in each group.The rats'skin was dividied into two areas by spine.The left side was used as control,and the right side was experiment side,which was treated with RF by viemedic RF ASC011C for five times at interval of seven days,and ten minutes in each time.The content of collagen in the dermis was measured by immunohistochemical method,the thickness of collagen bundles in the dermis was measured under HE staining and the quantity of collagen fibers was compared by collagen fibers staining.Results Epidermis was more smooth,the collagen fiber was thicker and the content of fibroblasts,collagen was higher in experiment side.Image quantitative analysis showed significant improvement in area density of collagen fibers.Conclusions RF is a very safe and effective method for non-ablative photorejuvnation.
