中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2010年
8期
574-577
,共4页
戴维德%范智慧%陈敏华%河福金%李洪民
戴維德%範智慧%陳敏華%河福金%李洪民
대유덕%범지혜%진민화%하복금%리홍민
射频消融%大鼠%肝肿瘤%树突状细胞%巨噬细胞炎性蛋白-1α%迁移
射頻消融%大鼠%肝腫瘤%樹突狀細胞%巨噬細胞炎性蛋白-1α%遷移
사빈소융%대서%간종류%수돌상세포%거서세포염성단백-1α%천이
Radiofrequency ablation%Rats%Liver tumors%Dendritic cells%Macrophage inflammatory protein-1α%Migration
目的 研究射频消融(RFA)治疗大鼠肝肿瘤前、后肝脏局部组织内单个核细胞中OX-62、OX-6及CD86阳性表达率及其相关趋化因子巨噬细胞炎性蛋白1α(MIP-1α)的变化,探讨RFA对肝脏局部组织内树突状细胞分化迁移的影响.方法 取60只正常Sprague Dawley(SD)大鼠制作Walker-256肝肿瘤模型后,分为RFA后7 d组、RFA后14 d组和荷瘤对照组,每组20只.RFA后7 d组、RFA后14 d组分别于RFA处理后第7天及第14天处死,对照组不做RFA处理即处死.取消融灶周边(对照组取肿瘤周边)0.5 cm范围内肝组织,采用Ficoll密度梯度离心法分离出单个核细胞,应用流式细胞仪检测单个核细胞OX-62、OX-6及CD86表达水平.采用酶联免疫吸附测定(ELISA)法检测肿瘤周边或消融灶周边0.5 cm范围内肝组织内MIP-1α浓度.结果 荷瘤对照组肿瘤周边肝组织内单个核细胞中有(15.29±4.59)%表达OX-6,RFA后7 d组和RFA后14 d组OX-6阳性表达率分别为(34.20±11.62)%、(39.18±9.14)%,与荷瘤对照组相比差异均有统计学意义(P＜0.05).荷瘤对照组肿瘤周边肝组织内单个核细胞中有(18.91±4.58)%表达OX-62,RFA后7 d组和RFA后14 d组OX-62阳性表达率分别为(24.49±4.45)%、(22.77±3.50)%,RFA后7 d组与荷瘤对照组相比差异有统计学意义(P＜0.05).荷瘤对照组肿瘤周边肝组织内单个核细胞中有(66.29±17.69)%表达CD86,RFA后7 d组CD86阳性表达率为(55.29±10.69)%,RFA后14 d组为(55.93±12.64)%,2组与荷瘤对照组比较差异均无统计学意义(P＞0.05).荷瘤对照组肿瘤周边肝组织内MIP-1α浓度为(232.92±54.58)pg/ml,RFA后7 d组和RFA后14 d组分别为(499.38±15.14)pg/ml、(495.90±9.94)pg/ml,与荷瘤对照组相比差异均有统计学意义(P＜0.05).结论 RFA治疗后荷瘤大鼠肝脏MIP-1α表达水平增高,促进树突状细胞的迁移,而肝脏单个核细胞OX-62、OX-6及CD86阳性表达率的变化也反映出这类细胞分化数量增多,有利于提高机体局部抗原提呈能力及宿主抗肿瘤免疫应答的恢复.
目的 研究射頻消融(RFA)治療大鼠肝腫瘤前、後肝髒跼部組織內單箇覈細胞中OX-62、OX-6及CD86暘性錶達率及其相關趨化因子巨噬細胞炎性蛋白1α(MIP-1α)的變化,探討RFA對肝髒跼部組織內樹突狀細胞分化遷移的影響.方法 取60隻正常Sprague Dawley(SD)大鼠製作Walker-256肝腫瘤模型後,分為RFA後7 d組、RFA後14 d組和荷瘤對照組,每組20隻.RFA後7 d組、RFA後14 d組分彆于RFA處理後第7天及第14天處死,對照組不做RFA處理即處死.取消融竈週邊(對照組取腫瘤週邊)0.5 cm範圍內肝組織,採用Ficoll密度梯度離心法分離齣單箇覈細胞,應用流式細胞儀檢測單箇覈細胞OX-62、OX-6及CD86錶達水平.採用酶聯免疫吸附測定(ELISA)法檢測腫瘤週邊或消融竈週邊0.5 cm範圍內肝組織內MIP-1α濃度.結果 荷瘤對照組腫瘤週邊肝組織內單箇覈細胞中有(15.29±4.59)%錶達OX-6,RFA後7 d組和RFA後14 d組OX-6暘性錶達率分彆為(34.20±11.62)%、(39.18±9.14)%,與荷瘤對照組相比差異均有統計學意義(P＜0.05).荷瘤對照組腫瘤週邊肝組織內單箇覈細胞中有(18.91±4.58)%錶達OX-62,RFA後7 d組和RFA後14 d組OX-62暘性錶達率分彆為(24.49±4.45)%、(22.77±3.50)%,RFA後7 d組與荷瘤對照組相比差異有統計學意義(P＜0.05).荷瘤對照組腫瘤週邊肝組織內單箇覈細胞中有(66.29±17.69)%錶達CD86,RFA後7 d組CD86暘性錶達率為(55.29±10.69)%,RFA後14 d組為(55.93±12.64)%,2組與荷瘤對照組比較差異均無統計學意義(P＞0.05).荷瘤對照組腫瘤週邊肝組織內MIP-1α濃度為(232.92±54.58)pg/ml,RFA後7 d組和RFA後14 d組分彆為(499.38±15.14)pg/ml、(495.90±9.94)pg/ml,與荷瘤對照組相比差異均有統計學意義(P＜0.05).結論 RFA治療後荷瘤大鼠肝髒MIP-1α錶達水平增高,促進樹突狀細胞的遷移,而肝髒單箇覈細胞OX-62、OX-6及CD86暘性錶達率的變化也反映齣這類細胞分化數量增多,有利于提高機體跼部抗原提呈能力及宿主抗腫瘤免疫應答的恢複.
목적 연구사빈소융(RFA)치료대서간종류전、후간장국부조직내단개핵세포중OX-62、OX-6급CD86양성표체솔급기상관추화인자거서세포염성단백1α(MIP-1α)적변화,탐토RFA대간장국부조직내수돌상세포분화천이적영향.방법 취60지정상Sprague Dawley(SD)대서제작Walker-256간종류모형후,분위RFA후7 d조、RFA후14 d조화하류대조조,매조20지.RFA후7 d조、RFA후14 d조분별우RFA처리후제7천급제14천처사,대조조불주RFA처리즉처사.취소융조주변(대조조취종류주변)0.5 cm범위내간조직,채용Ficoll밀도제도리심법분리출단개핵세포,응용류식세포의검측단개핵세포OX-62、OX-6급CD86표체수평.채용매련면역흡부측정(ELISA)법검측종류주변혹소융조주변0.5 cm범위내간조직내MIP-1α농도.결과 하류대조조종류주변간조직내단개핵세포중유(15.29±4.59)%표체OX-6,RFA후7 d조화RFA후14 d조OX-6양성표체솔분별위(34.20±11.62)%、(39.18±9.14)%,여하류대조조상비차이균유통계학의의(P＜0.05).하류대조조종류주변간조직내단개핵세포중유(18.91±4.58)%표체OX-62,RFA후7 d조화RFA후14 d조OX-62양성표체솔분별위(24.49±4.45)%、(22.77±3.50)%,RFA후7 d조여하류대조조상비차이유통계학의의(P＜0.05).하류대조조종류주변간조직내단개핵세포중유(66.29±17.69)%표체CD86,RFA후7 d조CD86양성표체솔위(55.29±10.69)%,RFA후14 d조위(55.93±12.64)%,2조여하류대조조비교차이균무통계학의의(P＞0.05).하류대조조종류주변간조직내MIP-1α농도위(232.92±54.58)pg/ml,RFA후7 d조화RFA후14 d조분별위(499.38±15.14)pg/ml、(495.90±9.94)pg/ml,여하류대조조상비차이균유통계학의의(P＜0.05).결론 RFA치료후하류대서간장MIP-1α표체수평증고,촉진수돌상세포적천이,이간장단개핵세포OX-62、OX-6급CD86양성표체솔적변화야반영출저류세포분화수량증다,유리우제고궤체국부항원제정능력급숙주항종류면역응답적회복.
Objective To study changes in the expression levels of OX-62, OX-6 and CD86 of mononuclear cells and the related chemotatic factor macrophage inflammatory protein-1α (MIP-1α) in the livers of rats with Walker-256 tumors treated with radiofrequency ablation (RFA) and to elucidate the influence of RFA on differentiation and migration of liver dendritic cells(DCs).Methods Walker-256 liver tumors were induced in 60 SpragueDawley rats by implanting tumor particles. These rats were randomly divided equally into three groups from which liver tissue around the local area of the tumor was sampled at 7 d and 14 d after RFA. The mononuclear liver cells were separated with Ficoll density gradient centrifugation. The expression levels of OX-62, OX-6 and CD86 in the mononuclear cells were analyzed with flow cytometry. The expression level of MIP-1α in the liver tissue was detected by enzyme-linked immunosorbent assay (ELISA). Results The average expression of OX-6 in the control rats was 15.29 ±4.59% and those 7 d and 14 d after RFA were 34.20±11.62% and 39.18±9.14% respectively. The difference between the two RFA groups and the control group was statistically significant. The average expression rate of OX-62 in the control rats was 18.91±4.58% and those 7 d and 14 d after RFA were 24.49±4.45% and 22.77 ± 3.50% respectively. The difference between the 7 d group and the control group was significant. The average expression rate of CD86 in the control rats was 66.29±17.69% and those 7 d and 14 d after RFA were 55.29±10.69% and 55.93±12.64% respectively. These differences between both RFA groups and the controls group were not significant. The average expression level of MIP-1 α around the tumors was 232.92±54.58 pg/ml in the controls and 499.38±15.14 pg/ml and 495.90±9.94 pg/ml 7d and 14 d after RFA respectively. These differences from the controls were both statistically significant. Conclusion The expression of MIP-1α around the tumors was elevated after RFA, which could promote the migration of DCs. The changes in the expression of OX-62, OX-6 and CD86 also could reflect increased DC differentiation, which could improve local antigen-presenting capacity to a certain extent and recovery of host anti-tumor immune response.
