CAJ | 학술논문

目的研究运动训练对痴呆小鼠空间记忆功能的影响及其可能分子机制.方法将30只昆明小鼠分为正常对照组(对照组)、痴呆组和痴呆加运动训练组(运动训练组),每组10只.痴呆组与运动训练组腹腔注射东莨菪碱制作痴呆模型,运动训练组在注射东莨菪碱30 min后利用滚筒式网状训练器进行运动训练6周.检测各组小鼠水迷宫的逃避潜伏期,Western印迹检测海马脑源性神经营养因子(BDNF)和蛋白激酶( Akt)磷酸化的表达,并检测海马一氧化氮(NO)和超氧化物歧化酶(SOD)的活性.结果腹腔注射东莨菪碱成功模拟小鼠的记忆缺失模型,表现为水迷宫逃避潜伏期延长.与痴呆组小鼠相比,运动训练组小鼠的水迷宫逃避潜伏期缩短(P＜0.01),且海马Akt的磷酸化和BDNF的表达增加(P＜0.05)；痴呆小鼠较对照组小鼠海马NO含量增加而SOD活性降低(P＜0.01),而运动训练部分逆转了上述现象.结论运动训练能显著提高注射东莨菪碱所致痴呆小鼠的空间记忆能力,痴呆小鼠行为功能的改善可能与海马Akt的磷酸化和BDNF表达的增加、NO含量的降低和SOD活性的增加有关.
목적 연구운동훈련대치태소서공간기억공능적영향급기가능분자궤제.방법 장30지곤명소서분위정상대조조(대조조)、치태조화치태가운동훈련조(운동훈련조),매조10지.치태조여운동훈련조복강주사동랑탕감제작치태모형,운동훈련조재주사동랑탕감30 min후이용곤통식망상훈련기진행운동훈련6주.검측각조소서수미궁적도피잠복기,Western인적검측해마뇌원성신경영양인자(BDNF)화단백격매( Akt)린산화적표체,병검측해마일양화담(NO)화초양화물기화매(SOD)적활성.결과복강주사동랑탕감성공모의소서적기억결실모형,표현위수미궁도피잠복기연장.여치태조소서상비,운동훈련조소서적수미궁도피잠복기축단(P＜0.01),차해마Akt적린산화화BDNF적표체증가(P＜0.05)；치태소서교대조조소서해마NO함량증가이SOD활성강저(P＜0.01),이운동훈련부분역전료상술현상.결론 운동훈련능현저제고주사동랑탕감소치치태소서적공간기억능력,치태소서행위공능적개선가능여해마Akt적린산화화BDNF표체적증가、NO함량적강저화SOD활성적증가유관.
Objective To investigate the effects of exercise on memory impairment in demented mice and possible molecular mechanisms. Methods Thirty Kunming nice were divided into a normal control group,a dementia group and a dementia plus exercise training group (exercise training group),10 mice/group. Dementia models were established by intraperitoneal injection of scopolamine.Exercise training was begun 30 min after the injection and lasted for a 6-week period. A water maze test was performed after the exercise training. Brain-derived neurotrophic factor (BDNF) expression and protein kinase (Akt) phosphorylation in the hippocampus were detected by Western blot analysis.Nitric oxide (NO) content and superoxide dismutase (SOD) activity in the hippocampus were also measured. Results Dementia models were successfully induced as indicated by prolonged escape latency in the water maze test.After exercise training the escape latency had shortened significantly and hippocanpal BDNF expression and Akt phosphorylation had increased.NO content increased and SOD activity decreased after the injections,but these effects were partially reversed after exercise training. Conclusions Exercise can improve spatial memory significantly after scopolanine-induced dementia in mice.The increased hippocampal BDNF expression and Akt phosphorylation,increased SOD activity and decreased NO content may partially explain the behavior improvement induced by exercise.