CAJ | 학술논문

目的检测胃癌中雌二醇(E2)、睾酮(T)、17β-羟基类固醇脱氢酶2(17β-HSD2)和17β-羟基类固醇脱氢酶5(17β-HSD5)的表达,探讨其之间的关系及作用.方法应用组织芯片和免疫组织化学技术检测56对样本(胃癌和癌旁正常组织)中E2和T蛋白表达,用逆转录-聚合酶链反应(RT-PCR)方法检测56对样本中17β-HSD2和17β-HSD5 mRNA表达.结果 (1)胃癌组织中E2蛋白的相对表达量(0.2381±0.0718)远高于癌旁正常组织(0.1832±0.0628),两者比较差异有统计学意义P＜0.05；与之相反,胃癌组织中17β-HSD2 mRNA的相对表达量(0.8065±0.3797)远低于癌旁正常组织(0.9788±0.2325),两者比较差异有统计学意义(P＜0.05)；在胃痈组织中E2和17β-HSD2之间呈负相关关系(r=0.294,P＜0.05)；(2)胃癌组织中E2蛋白相对表达量有淋巴结转移(0.2532±0.0667)、无淋巴结转移(0.1883±0.0673)间比较和TNM分期中Ⅰ(0.1813±0.0686)、Ⅱ(0.2111±0.0633)、Ⅲ(0.2534±0.0727)、Ⅳ(0.2595±0.0540)间比较均差异有统计学意义(P＜0.05)；(3)胃癌组织中17β-HSD2 mRNA相对表达量男(0.8645±0.3415)及女(0.6145±0.4479)之间、分化好(0.6717±0.4182)、分化差(0.8714±0.3613)间比较差异均有统计学意义(P＜0.05).结论胃癌发生发展与E2蛋白表达增强和17β-HSD2 mRNA转录减弱明显相关.
목적 검측위암중자이순(E2)、고동(T)、17β-간기류고순탈경매2(17β-HSD2)화17β-간기류고순탈경매5(17β-HSD5)적표체,탐토기지간적관계급작용.방법 응용조직심편화면역조직화학기술검측56대양본(위암화암방정상조직)중E2화T단백표체,용역전록-취합매련반응(RT-PCR)방법검측56대양본중17β-HSD2화17β-HSD5 mRNA표체.결과 (1)위암조직중E2단백적상대표체량(0.2381±0.0718)원고우암방정상조직(0.1832±0.0628),량자비교차이유통계학의의P＜0.05；여지상반,위암조직중17β-HSD2 mRNA적상대표체량(0.8065±0.3797)원저우암방정상조직(0.9788±0.2325),량자비교차이유통계학의의(P＜0.05)；재위옹조직중E2화17β-HSD2지간정부상관관계(r=0.294,P＜0.05)；(2)위암조직중E2단백상대표체량유림파결전이(0.2532±0.0667)、무림파결전이(0.1883±0.0673)간비교화TNM분기중Ⅰ(0.1813±0.0686)、Ⅱ(0.2111±0.0633)、Ⅲ(0.2534±0.0727)、Ⅳ(0.2595±0.0540)간비교균차이유통계학의의(P＜0.05)；(3)위암조직중17β-HSD2 mRNA상대표체량남(0.8645±0.3415)급녀(0.6145±0.4479)지간、분화호(0.6717±0.4182)、분화차(0.8714±0.3613)간비교차이균유통계학의의(P＜0.05).결론 위암발생발전여E2단백표체증강화17β-HSD2 mRNA전록감약명현상관.
Objective To investigate the expression of estradiol (E2),testosterone (T),estrogen receptor ( ER),androgen receptor (AR),17β-hydroxysteroid dehydrogenase2 (17β-HSD2) and 17β-hydroxysteroid dehydrogenase5 (17β-HSD5) in gastric carcinomas and their relationship. Methods Tissue microarray technology and immunohistochemical Elivision plus method were used to detect the expression of E2,T protein in 56 pairs of samples of gastric carcinoma and normal gastric tissue. The mRNA transcription levels of 17β-HSD2 and 17β-HSD5 were measured by reverse transcription-polymerase chain reaction (RT-PCR). Results ( 1 ) The E2 protein expression levels in gastric carcinoma tissues (0. 2381±0. 0718) was significantly higher than in normal gastric tissues (0. 1832 ±0. 0628 ,P ＜0. 05),on the contrary,the 17β-HSD2 mRNA transcription level in gastric carcinoma tissues (0. 8065 0. 3797) was significantly lower than in normal gastric tissues (0. 9788 0. 2325,P ＜ 0. 05 ),a negative correlation between them (r =0. 294,P ＜0. 05);(2) The E2 protein level was higher in gastric carcinoma tissues with lymph node metastasis ( 0. 2532 ± 0. 0667 ) than in those without lymph node metastasis ( 0. 1883 ±0. 0673,P ＜ 0. 05 ). The expression of E2 protein in TNM stages Ⅰ ,Ⅱ ,Ⅲ,Ⅳ respectively was (0. 1813±0.0686),(0.2111 ±0.0633),(0.2534 ±0.0727),(0.2595 ±0.0540);(3) The 17β-HSD2mRNA transcription level was higher in gastric carcinoma tissues of men (0. 8645 0. 3415) than in those of women (0. 6145 0. 4479,P ＜0. 05) and the same positive relationship was observed between the well differentiation and the poorer differentiation. Conclusion The tumorigenesis and progression of gastric carcinoma has an important correlation with up-regulated E2 protein expression level and down-regulated 17β-HSD2 mRNA transcription level.