CAJ | 학술논문

目的观察肿瘤转移抑制基因nm23的表达产物二磷酸核苷激酶（nucleoside diphosphat kinase，NDPK）在裸小鼠玻璃体腔视网膜母细胞瘤（retinoblastoma, RB）移植瘤中的表达状态，探讨nm23基因的表达产物NDPK（nm23／NDPK）与RB的发生发展及浸润生长的相关性。方法对20例裸小鼠玻璃体RB移植瘤进行nm23／NDPK表达的免疫组织化学染色，以正常视网膜组织标本作对照，比较RB移植瘤眼内生长期（Ⅰ～Ⅲ级）及蔓延、扩展浸润期（Ⅳ～Ⅴ级）nm23／NDPK的表达情况。结果正常视网膜组织nm23蛋白表达阴性。20例RB移植瘤nm23／NDPK表达阳性，阳性细胞数为48．73±2．37，阳性表达率为100%；眼内生长期（Ⅰ～Ⅲ级）与蔓延、扩展浸润期（Ⅳ～Ⅴ级） nm23/NDPK阳性表达细胞数差异无显著性的意义(P＞0．05)。结论 nm23基因不具有抑制RB移植瘤蔓延浸润的功能，而是在其发生发展过程中起一定作用。
목적관찰종류전이억제기인nm23적표체산물이린산핵감격매（nucleoside diphosphat kinase，NDPK）재라소서파리체강시망막모세포류（retinoblastoma, RB）이식류중적표체상태，탐토nm23기인적표체산물NDPK（nm23／NDPK）여RB적발생발전급침윤생장적상관성。방법대20례라소서파리체RB이식류진행nm23／NDPK표체적면역조직화학염색，이정상시망막조직표본작대조，비교RB이식류안내생장기（Ⅰ～Ⅲ급）급만연、확전침윤기（Ⅳ～Ⅴ급）nm23／NDPK적표체정황。결과정상시망막조직nm23단백표체음성。20례RB이식류nm23／NDPK표체양성，양성세포수위48．73±2．37，양성표체솔위100%；안내생장기（Ⅰ～Ⅲ급）여만연、확전침윤기（Ⅳ～Ⅴ급） nm23/NDPK양성표체세포수차이무현저성적의의(P＞0．05)。결론 nm23기인불구유억제RB이식류만연침윤적공능，이시재기발생발전과정중기일정작용。
Purpose　To investigate nucleoside diphosphate kinase (NDPK ) expression of tumor metastasis suppressor gene nm23 in heterotransplanted model of retinoblastoma(RB) in nude mice，and analyse the correlation between the expression of nm23 gene and the formation and progression of heterotransplanted RB．　Methods　SP immunohistochemical method was used to detect the expression of nm23 gene product NDPK in 20 tumors of heterotransplanted RB model and normal retinal tissue．　Results　The negative staining of nm23/ NDPK was found in normal retinal tissue , whereas 100% expression rate in RB tumors with positive number of 48．73±2．37． No statistical significance of the expression of nm23/ NDPK was observed between the intraocular growth phase （I～Ⅲ grade） and invasive phase （Ⅳ～Ⅴ grade） in heterotransplanted RB tumors．　Conclusion　The function of nm23 gene as a tumor metastasis suppressor in heterotransplanted RB tumors was less prominent ,but it may play a role in carcinogenesis and progrssion of RB and may predict poor prognosis．