CAJ | 학술논문

目的评价姜黄素对大鼠糖尿病神经病理性痛(DNP)的效应.方法雄性SD大鼠48只,随机分为6组(n=8):正常对照组(C组)、DNP组(D组)、DNP+二甲基亚砜组(DD组)和DNP+姜黄素50、100、200 mg/kg组(DC50组、DC100组和DC200组).D组、DD组、DC50组、DC100组和DC200组采用腹腔注射链唑霉素75 mg/kg的方法制备糖尿病神经病理性痛模型,注射链唑霉素后14 d开始腹腔注射二甲基亚砜(姜黄素的溶媒)或相应剂量姜黄素,1次/d,连续2周.分别造模前2 d、注射链唑霉素后14 d、姜黄素给药1、3、7、14 d时测定机械缩足痛阚(MWT)和热缩足潜伏期(TWL),最后一次测定痛阈后处死大鼠,测定脊髓背角和背根神经节细胞核磷酸化JNK(p-JNK)和NF-кB p65的表达水平.结果与C组相比,D组各时点MWT降低,TWL缩短,脊髓背角和背根神经节细胞核p-JNK和NF-кB p65表达上调(P<0.05).与D组相比,DC50组、DC100组和DC200组姜黄素给药期间MWT升高,TWL延长,且与剂量有关,脊髓背角和背根神经节细胞核p-JNK和NF-кB p65表达下调(P<0.05).结论姜黄素可减轻大鼠糖尿病神经病理性痛,其机制可能与抑制脊髓背角和背根神经节JNK和NF-кB的激活有关.
목적 평개강황소대대서당뇨병신경병이성통(DNP)적효응.방법 웅성SD대서48지,수궤분위6조(n=8):정상대조조(C조)、DNP조(D조)、DNP+이갑기아풍조(DD조)화DNP+강황소50、100、200 mg/kg조(DC50조、DC100조화DC200조).D조、DD조、DC50조、DC100조화DC200조채용복강주사련서매소75 mg/kg적방법 제비당뇨병신경병이성통모형,주사련서매소후14 d개시복강주사이갑기아풍(강황소적용매)혹상응제량강황소,1차/d,련속2주.분별조모전2 d、주사련서매소후14 d、강황소급약1、3、7、14 d시측정궤계축족통감(MWT)화열축족잠복기(TWL),최후일차측정통역후처사대서,측정척수배각화배근신경절세포핵린산화JNK(p-JNK)화NF-кB p65적표체수평.결과 여C조상비,D조각시점MWT강저,TWL축단,척수배각화배근신경절세포핵p-JNK화NF-кB p65표체상조(P<0.05).여D조상비,DC50조、DC100조화DC200조강황소급약기간MWT승고,TWL연장,차여제량유관,척수배각화배근신경절세포핵p-JNK화NF-кB p65표체하조(P<0.05).결론 강황소가감경대서당뇨병신경병이성통,기궤제가능여억제척수배각화배근신경절JNK화NF-кB적격활유관.
Objective To evaluate the effect of curcumin on diabetic neuropathic pain (DNP) in rats. Methods Forty-eight male SD rats weighing 200-230 g were randomly divided into 6 groups ( n = 8 each) : group Ⅰ normal control (group C); group Ⅱ DNP (group D) ; group Ⅲ DNP+ DMSO (solvent of curcumin) (group DD) and group Ⅳ , Ⅴ , Ⅵ DNP + curcumin 50, 100 or 200 mg/kg ( group DC50, 100, 200 ). Diabetes was induced with intraperitoneal (IP) streptozocin 75 mg/kg in group Ⅱ -Ⅵ. Curcumin 50, 100 and 200 mg/kg were given IP once a day for 14 consecutive days starting from 14 d after streptozocin in group Ⅳ , Ⅴ and Ⅵ respectively. Venous blood samples were taken before and 72 h after IP streptosocin for determination of blood glucose level. Successful induction of diabetes was defined as blood glucose > 14.6 mmol/L. Mechanical paw withdrawal threshold to yon Frey stimuli (MWT) and paw withdrawal latency to thermal nociceptive stimulus (TWL) were measured 2 d before and 14 d after IP streptesocin and on the 1, 3, 7, 14 d of curcumin administration. Animals with pain threshold measured at 14 d after IP streptozocin decreasing by less than 15% of the baseline were excluded from the study. The animals were sacrificed after the last pain threshold measurement and the lumbar segment of the spinal cord and p65 was significantly higher in group D than in group C ( P < 0.05). Curcumin administration significantly reduced thermal and mechanical hyperalgesia induced by diabetes and down-regulated the expression of p-JNK and horn and DRG.