中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2012年
5期
314-319
,共6页
胃肿瘤%DNA甲基化%核心结合因子α3亚基
胃腫瘤%DNA甲基化%覈心結閤因子α3亞基
위종류%DNA갑기화%핵심결합인자α3아기
Stomach neoplasms%DNA methylation%Core binding factor alpha 3 subunit
目的 通过研究Runx3基因CpG岛甲基化对胃癌病变的影响,探讨甲基化特异性聚合酶链反应(MSP)法与焦磷酸测序(PS)法对Runx3基因甲基化检测的特异性.方法 选取150例胃癌患者胃切除标本及50例无癌变胃黏膜标本,以MSP法和PS法同时检测其Runx3启动子的甲基化程度.结果 与正常胃黏膜相比,胃癌组织Runx3的甲基化程度差异有显著性意义(MSP法:67.3％比40.0％,P=0.002；PS法:76.0％比30.0％,P＜0.01).MSP法显示Runx3甲基化只与肿瘤大小相关(P＜0.05).而PS法分析显示进展期胃癌的Runx3甲基化率高于早期胃癌(P＜0.05)；且其甲基化状态与肿瘤的大小(P =0.004)、Lauren分型(P=0.043)、浸润的深度(P＜0.01)、淋巴结转移(P=0.021)及肿瘤TNM临床分期(P=0.045)等临床病理特征之间存在相关性.结论 Runx3启动子甲基化与胃癌临床病理特征包括肿瘤大小、Lauren分型、浸润的深度、淋巴结转移以及肿瘤TNM临床分期密切相关；与MSP法相比,PS法敏感性更高,且检测结果与临床病理学结果之间有显著相关性,对于胃癌的早期诊断和治疗有着重要意义.
目的 通過研究Runx3基因CpG島甲基化對胃癌病變的影響,探討甲基化特異性聚閤酶鏈反應(MSP)法與焦燐痠測序(PS)法對Runx3基因甲基化檢測的特異性.方法 選取150例胃癌患者胃切除標本及50例無癌變胃黏膜標本,以MSP法和PS法同時檢測其Runx3啟動子的甲基化程度.結果 與正常胃黏膜相比,胃癌組織Runx3的甲基化程度差異有顯著性意義(MSP法:67.3％比40.0％,P=0.002；PS法:76.0％比30.0％,P＜0.01).MSP法顯示Runx3甲基化隻與腫瘤大小相關(P＜0.05).而PS法分析顯示進展期胃癌的Runx3甲基化率高于早期胃癌(P＜0.05)；且其甲基化狀態與腫瘤的大小(P =0.004)、Lauren分型(P=0.043)、浸潤的深度(P＜0.01)、淋巴結轉移(P=0.021)及腫瘤TNM臨床分期(P=0.045)等臨床病理特徵之間存在相關性.結論 Runx3啟動子甲基化與胃癌臨床病理特徵包括腫瘤大小、Lauren分型、浸潤的深度、淋巴結轉移以及腫瘤TNM臨床分期密切相關；與MSP法相比,PS法敏感性更高,且檢測結果與臨床病理學結果之間有顯著相關性,對于胃癌的早期診斷和治療有著重要意義.
목적 통과연구Runx3기인CpG도갑기화대위암병변적영향,탐토갑기화특이성취합매련반응(MSP)법여초린산측서(PS)법대Runx3기인갑기화검측적특이성.방법 선취150례위암환자위절제표본급50례무암변위점막표본,이MSP법화PS법동시검측기Runx3계동자적갑기화정도.결과 여정상위점막상비,위암조직Runx3적갑기화정도차이유현저성의의(MSP법:67.3％비40.0％,P=0.002；PS법:76.0％비30.0％,P＜0.01).MSP법현시Runx3갑기화지여종류대소상관(P＜0.05).이PS법분석현시진전기위암적Runx3갑기화솔고우조기위암(P＜0.05)；차기갑기화상태여종류적대소(P =0.004)、Lauren분형(P=0.043)、침윤적심도(P＜0.01)、림파결전이(P=0.021)급종류TNM림상분기(P=0.045)등림상병리특정지간존재상관성.결론 Runx3계동자갑기화여위암림상병리특정포괄종류대소、Lauren분형、침윤적심도、림파결전이이급종류TNM림상분기밀절상관；여MSP법상비,PS법민감성경고,차검측결과여림상병이학결과지간유현저상관성,대우위암적조기진단화치료유착중요의의.
Objective To investigate the role of Runx3 gene CpG island methylation in the development of human gastric carcinoma.Methods A total of 150 tumor specimens from patients with gastric carcinoma and 50 normal tissue specimens were selected. Methylation specific PCR (MSP) and pyrosequencing (PS) were used to detect the methylation status of Runx3 gene promoter. Results Compared to normal tissue samples,a significant increase of CpG island methylation status of Runx3 gene was observed in gastric carcinomas ( MSP:67.3％ vs.40.0％,P =0.002 ; PS:76.0％ vs.30.0％,P ＜0.01).Runx3 gene methylation was only related to tumor size ( P ＜ 0.05 ) based on MSP analysis.PS test however showed that the extent of methylation of Runx3 gene was related to the tumor size ( P =0.004 ),Lauren's classification ( P =0.043 ),depth of invasion ( P ＜ 0.01),lymph node metastasis ( P =0.021)and TNM staging ( P =0.045 ).Conclusions Methylation status of Runx3 gene detectable by PS is closely correlated with clinicopathological parameters of gastric carcinoma, including tumor size, Lauren' s classification,depth of invasion,lymph node metastasis and TNM staging.PS is more sensitive than MSP in the detection of Runx3 gene methylation,which may serve as an important marker for early diagnosis and treatment of gastric carcinoma.
