CAJ | 학술논문

采用正相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、反相制备液相色谱等手段进行分离纯化,并通过1H-NMR、13C-NMR等波谱技术进行结构鉴定,结果从乙酸乙酯层中分离得到了6个化合物,分别鉴定为bulbocol（1）,coelonin（2）,β-胡萝卜苷（3）,间羟基苯甲酸（4）,5-羟甲基糠醛（5）,3-羟基苯丙酸（6）,其中化合物3、4、5、6为首次从该植物中分离得到.
채용정상규효주색보、포취당응효Sephadex LH-20、반상제비액상색보등수단진행분리순화,병통과1H-NMR、13C-NMR등파보기술진행결구감정,결과종을산을지층중분리득도료6개화합물,분별감정위bulbocol（1）,coelonin（2）,β-호라복감（3）,간간기분갑산（4）,5-간갑기강철（5）,3-간기분병산（6）,기중화합물3、4、5、6위수차종해식물중분리득도.
The chemical constituents of the medicinal plant,Pleione bulbocodioides,was extacted and isolated by repeated column chromatography with silica gel,Sephadex LH-2 and Pre-HPLC.Six compounds were isolated from the EtOAc extract.Their structures were elucidated as bulbocol（1）,coelonin（2）,β-daucosterol（3）,3-hydroxybenzcic acid（4）,5-hydroxymethyl furfural（5）,3-hydroxyphenylpropionic acid（6） by analysis of spectroscopic data（1H-NMR,13C-NMR,）.Compounds 3,4,5,6 were isolated from the plant extract of Pleione bulbocodioides for the first time.