陕西师范大学学报:自然科学版
陝西師範大學學報:自然科學版
협서사범대학학보:자연과학판
Journal of Shaanxi Normal University: Nat Sci Ed
2012年
1期
100-103
,共4页
熊正英%刘海波%池爱平%王棣%强永浩%王娟%刘厚宇
熊正英%劉海波%池愛平%王棣%彊永浩%王娟%劉厚宇
웅정영%류해파%지애평%왕체%강영호%왕연%류후우
锁阳多糖%血清酶%尿蛋白%运动能力%鼠
鎖暘多糖%血清酶%尿蛋白%運動能力%鼠
쇄양다당%혈청매%뇨단백%운동능력%서
Cynomrium songaricum Rupr polysaccharide(CSRP)%serum enzyme%urinary protein%exercise capacity%rats
探讨锁阳多糖(Cynomrium songaricum Rupr polysaccharide,CSRP)对运动训练大鼠骨骼肌、心、肝、肾等组织细胞及线粒体的保护作用,为CSRP在运动医学中的应用提供实验依据.采用分光光度法测定安静对照组、运动训练组和运动+CSRP组大鼠血清琥珀酸脱氢酶(succinatedehydrogenase,SDH)、肌酸激酶(creatine kinase,CK)、乳酸脱氢酶酶(lactate dehydrogenase,LDH)、丙氨酸转氨酶(alanine transaminase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)活性和尿液总蛋白(total protein,TP)、尿液白蛋白(albumin,Alb)和尿液β2-微球蛋白(β2-microglobulin,β2-MC)含量等相关生物化学指标;跑台法测定运动训练组和运动+CSRP组大鼠运动至力竭的时间.结果表明:运动+CSRP组血清SDH、CK、LDH活性显著低于运动对照组(P〈0.05),ALT、AST活性显著低于运动对照组(P〈0.05),TP、Alb和β2-MC含量显著低于运动对照组(P〈0.05);运动+锁阳多糖组大鼠运动至力竭的时间与运动对照组比较明显延长(P〈0.05).表明CSRP可以降低血清SDH、CK、LDH、ALT、AST活性和TP、Alb和β2-MC含量,保护运动训练大鼠骨骼肌、心、肝、肾等不同组织细胞和线粒体的结构,维持其正常功能,可延长大鼠跑台运动至力竭时间,具有抗疲劳作用.
探討鎖暘多糖(Cynomrium songaricum Rupr polysaccharide,CSRP)對運動訓練大鼠骨骼肌、心、肝、腎等組織細胞及線粒體的保護作用,為CSRP在運動醫學中的應用提供實驗依據.採用分光光度法測定安靜對照組、運動訓練組和運動+CSRP組大鼠血清琥珀痠脫氫酶(succinatedehydrogenase,SDH)、肌痠激酶(creatine kinase,CK)、乳痠脫氫酶酶(lactate dehydrogenase,LDH)、丙氨痠轉氨酶(alanine transaminase,ALT)、天鼕氨痠轉氨酶(aspartate aminotransferase,AST)活性和尿液總蛋白(total protein,TP)、尿液白蛋白(albumin,Alb)和尿液β2-微毬蛋白(β2-microglobulin,β2-MC)含量等相關生物化學指標;跑檯法測定運動訓練組和運動+CSRP組大鼠運動至力竭的時間.結果錶明:運動+CSRP組血清SDH、CK、LDH活性顯著低于運動對照組(P〈0.05),ALT、AST活性顯著低于運動對照組(P〈0.05),TP、Alb和β2-MC含量顯著低于運動對照組(P〈0.05);運動+鎖暘多糖組大鼠運動至力竭的時間與運動對照組比較明顯延長(P〈0.05).錶明CSRP可以降低血清SDH、CK、LDH、ALT、AST活性和TP、Alb和β2-MC含量,保護運動訓練大鼠骨骼肌、心、肝、腎等不同組織細胞和線粒體的結構,維持其正常功能,可延長大鼠跑檯運動至力竭時間,具有抗疲勞作用.
탐토쇄양다당(Cynomrium songaricum Rupr polysaccharide,CSRP)대운동훈련대서골격기、심、간、신등조직세포급선립체적보호작용,위CSRP재운동의학중적응용제공실험의거.채용분광광도법측정안정대조조、운동훈련조화운동+CSRP조대서혈청호박산탈경매(succinatedehydrogenase,SDH)、기산격매(creatine kinase,CK)、유산탈경매매(lactate dehydrogenase,LDH)、병안산전안매(alanine transaminase,ALT)、천동안산전안매(aspartate aminotransferase,AST)활성화뇨액총단백(total protein,TP)、뇨액백단백(albumin,Alb)화뇨액β2-미구단백(β2-microglobulin,β2-MC)함량등상관생물화학지표;포태법측정운동훈련조화운동+CSRP조대서운동지력갈적시간.결과표명:운동+CSRP조혈청SDH、CK、LDH활성현저저우운동대조조(P〈0.05),ALT、AST활성현저저우운동대조조(P〈0.05),TP、Alb화β2-MC함량현저저우운동대조조(P〈0.05);운동+쇄양다당조대서운동지력갈적시간여운동대조조비교명현연장(P〈0.05).표명CSRP가이강저혈청SDH、CK、LDH、ALT、AST활성화TP、Alb화β2-MC함량,보호운동훈련대서골격기、심、간、신등불동조직세포화선립체적결구,유지기정상공능,가연장대서포태운동지력갈시간,구유항피노작용.
Effect of Cynomrium songaricum Rupr polysaccharide(CSRP)on protection role to skeletal muscle,heart,liver,kidney and other tissue cells and mitochondria in exercise training rats,for CSRP application in sports medicine and provide experimental basis.Using spectrophotometry to measure serum succinate dehydrogenase succinate dehydrogenase(SDH)、 creatine kinase(CK)、 lactate dehydrogenase enzyme(LDH)、 alanine aminotransferase(ALT),aspartate aminotransferase(AST) activity and urine total protein(TP)、 urinary albumin(Alb) and urine fluid β2-microglobulin(β2-MC) content and other related biochemical indicators in rats of quiet control group,exercise training group and the exercise + CSRP group;treadmill exercise to measure exercise time to exhaustion in rats of training group and determined campaign + CSRP.The results showed: exercise + CSRP serum SDH、CK、LDH activity was significantly lower than the exercise control group(P0.05);exercise + CSRP serum ALT、 AST activity was significantly lower than the exercise control group(P0.05);exercise + CSRP group of TP、 Alb,and β2-MC was significantly lower than the exercise control group(P0.05);exercise + Cynomorium polysaccharide rats exercise and exercise time to exhaustion of the control group was significantly longer(P0.05).The results show that CSRP can reduce the serum SDH、 CK、 LDH、 ALT、 AST activity and TP、 Alb and β2-MC content,to protect rats muscle、heart、liver、kidney and other tissue cells and mitochondria of different structures,to maintain its normal Function,may be extended to exhaustive treadmill exercise time with antifatigue effect.