CAJ | 학술논문

将鸡子豆粉碎后,采用蒸馏水冷浸提取其中的植物血凝素（PHA）,浸提后的上清液经（NH4）2SO4沉淀,100KD分子量的中空纤维超滤膜超滤,然后取PHA上清液、（NH4）2SO4沉淀液、超滤液和浓缩液测定血凝活性,血凝效价依次为28、27、29、27。通过试验,笔者认为超滤法可作为PHA的初步纯化方法。
장계자두분쇄후,채용증류수랭침제취기중적식물혈응소（PHA）,침제후적상청액경（NH4）2SO4침정,100KD분자량적중공섬유초려막초려,연후취PHA상청액、（NH4）2SO4침정액、초려액화농축액측정혈응활성,혈응효개의차위28、27、29、27。통과시험,필자인위초려법가작위PHA적초보순화방법。
Phytohaemagglutinin was extracted by distilled water at 4 ℃ from crushed beans, and its supernatant was precipitated by（NH4）2SO4, ultrafiltrated by 100 KD molecular weight fiber membrane respectively. The supernatant solution,（NH4）2SO4 precipitation liquid, super and concentrated liquid of filtration were collected to test the hemagglutinin activity（HA）, and the HA titer of which were 28,27,29,27 respectively. The conclusion indicated that ultrafiltration could be used as a preliminary purification of PHA.