CAJ | 학술논문

以斑点叉尾鮰鱼皮为实验材料，选用乳酸从中提取胶原蛋白。通过单因素试验分别考察pH值、料液比和时间3个参数对胶原蛋白提取率的影响，在此基础上，利用Box—Benhnken中心组合试验设计和响应面分析法对乳酸提取阚鱼皮胶原蛋白条件进行优化。结果表明，乳酸提取鮰鱼皮胶原蛋白的优化工艺为pH2．3、料液比1：60（g／mL）、时间46h，在此条件下鮰鱼皮胶原蛋白的提取率达到46．28％。
이반점차미회어피위실험재료，선용유산종중제취효원단백。통과단인소시험분별고찰pH치、료액비화시간3개삼수대효원단백제취솔적영향，재차기출상，이용Box—Benhnken중심조합시험설계화향응면분석법대유산제취감어피효원단백조건진행우화。결과표명，유산제취회어피효원단백적우화공예위pH2．3、료액비1：60（g／mL）、시간46h，재차조건하회어피효원단백적제취솔체도46．28％。
This study was undertaken to optimize the acidic extraction of collagen from channel catfish （lctalurus punctatus） skin. Lactic acid was found to be a better solvent for extracting collagen from channel catfish skin than formic acid, acetic acid, citric acid and hydrochloric acid. Further, three process conditions affecting the extraction rate of collagen with lactic acid, such as pH value, solid-to-liquid ratio and extraction time, were optimized by one-factor-at-a-time method and Box-Benhnken experimental design combined with response surface methodology. The results indicated that the optimal conditions for collagen extraction were pH 2.3, solid-to-liquid ratio 1：60 （g/mL） and extraction time 46 h, under which, an extraction rate of 46.28% was obtained.