CAJ | 학술논문

研究纤维素酶降解壳聚糖生产壳寡糖的工艺条件，对影响壳聚糖降解的因素进行探讨，通过正交试验优化酶解反应工艺参数。结果表明：影响壳聚糖降解的主次膨序分别为反应时间〉纤维素酶和壳聚糖比例〉温度〉pH值：最佳酶解条件为温度40℃纤维索酶：壳聚糖=1：6，pH值5．0，反应时间8h。壳寡糖的得率为96．5％。
연구섬유소매강해각취당생산각과당적공예조건，대영향각취당강해적인소진행탐토，통과정교시험우화매해반응공예삼수。결과표명：영향각취당강해적주차팽서분별위반응시간〉섬유소매화각취당비례〉온도〉pH치：최가매해조건위온도40℃섬유색매：각취당=1：6，pH치5．0，반응시간8h。각과당적득솔위96．5％。
The production process of chito-oligosaccharide by cellulose enzyme degradation were researched. The influence racquet＇s ,of chito-oligosaccharide degradation were discussed. And the cellulose enzyme reaction process parameter was optimized tt,rough orthogonal experiment in this paper. The results showed that the sequence of influencing chito-oligosaceharide degradation was the reaction time〉 cellulose enzyme and chito-oligosaccharide proportion〉temperature〉pH. The best zymolysing conditions were temperature 40℃,cellulose enzyme： chito-oligosaccharide for 6, pH 5, reaction time 8 h. The chito-oligosaccharide output was 96.5% under the condition of optimization degradation conditions.