CAJ | 학술논문

[目的]研究甘蔗F2后代材料间是否存在遗传差异。[方法]采用11条引物对20份蔗茅杂种F2材料进行ISSR分析。[结果]共扩增出121条带,其中110条为多态性带,多态率达90.91%,说明栽培甘蔗与蔗茅杂交F2材料间存在着丰富的变异;供试F2代材料与其母本＂崖城89-9＂及父本＂昆明蔗茅＂间的平均遗传相似系数分别为0.71和0.54,且从聚类结果来看,各子代材料均与母本聚为一类,说明母本的遗传物质在子代材料中占绝对优势。[结论]该研究可为甘蔗创新种质材料在甘蔗育种中的应用提供依据。
[목적]연구감자F2후대재료간시부존재유전차이。[방법]채용11조인물대20빈자모잡충F2재료진행ISSR분석。[결과]공확증출121조대,기중110조위다태성대,다태솔체90.91%,설명재배감자여자모잡교F2재료간존재착봉부적변이;공시F2대재료여기모본＂애성89-9＂급부본＂곤명자모＂간적평균유전상사계수분별위0.71화0.54,차종취류결과래간,각자대재료균여모본취위일류,설명모본적유전물질재자대재료중점절대우세。[결론]해연구가위감자창신충질재료재감자육충중적응용제공의거。
[Objective] The aim was to explore the genetic differences among F2 progenies of sugarcane.[Method] 11 primers were used for ISSR analysis on 20 F2 hybrids of Erianthus fulvus.[Result] A total of 121 bands were amplified in the PCR reactions,among which 110 were polymorphic bands and the polymorphic rate was 90.91%,indicating that there were abundance genetic variation among F2 progenies derived from cross combinations between cultivated sugarcane and E.fulvus.The average genetic coefficients between Yacheng 89/9 and F1 progenies,E.fulvus and F1 progenies,were 0.71 and 0.54,respectively.The clustering result of F2 progenies showed that all the progenies clustered with female parent and suggested that the genetic material of female parent was predominated in F2 progenies.[Conclusion] The study could provide basis for the application of sugarcane innovation germplasm materials in the breeding of sugarcane.