CAJ | 학술논문

[目的]克隆水稻OsOle1基因并对其进行遗传转化。[方法]通过RT-PCR方法对OsOle1基因进行克隆,将克隆出的OsOle1基因连接到pCAMBIA1300上构建其超表达载体,并通过农杆菌介导对水稻愈伤进行了遗传转化。[结果]克隆出的OsOle1基因全长498bp,编码165个氨基酸,成功构建了其超表达载体Ub：：OsOe1-GUS,最终得到了转基因株系。[结论]为OsOle1基因的功能研究奠定了基础。
[목적]극륭수도OsOle1기인병대기진행유전전화。[방법]통과RT-PCR방법대OsOle1기인진행극륭,장극륭출적OsOle1기인련접도pCAMBIA1300상구건기초표체재체,병통과농간균개도대수도유상진행료유전전화。[결과]극륭출적OsOle1기인전장498bp,편마165개안기산,성공구건료기초표체재체Ub：：OsOe1-GUS,최종득도료전기인주계。[결론]위OsOle1기인적공능연구전정료기출。
[Objective] The cloning and transformation of rice OsOle1 gene were conducted in the research.[Method] OsOle1 gene was cloned by RT-PCR.The amplified OsOle1 was then ligased to pCAMBIA 1300 to construct GUS overexpression vector.Then the Agrobacterium-mediated method was used in rice callus transformation.[Result] The full-length of OsOle1 gene was 498 bp and it encoded 189 amino acids.The over-expression vector Ub：：OsOe1-GUS was prepared and transgenic plants were successfully obtained.[Conclusion] The transgenic lines laid the foundation for the function research of OsOle1.