CAJ | 학술논문

为研究表皮生长因子（EGF）和胰岛素（Insulin）对牛卵母细胞体外发育的影响以及体细胞共培养对体外受精胚胎体外发育的影响，将获取的牛卵泡卵母细胞在含有30μg，LEGF或50mg／L Insulin或30μg／LEGF＋50mg／L Insulin的成熟培养液中进行成熟培养，比较不同生长因子对牛卵母细胞体外成熟和体外受精的影响；将受精卵用颗粒细胞单层培养系统或牛输卵管上皮细胞单层培养系统进行体外培养，比较体细胞共培养系统对体外受精胚胎体外发育的影响。结果表明，成熟培养液中添加30μg／LEGF或者30μg／LEGF＋50mg／L Insulin可以显著提高卵母细胞的成熟率和受精后的卵裂率（P〈0．05）；输卵管上皮细胞共培养系统可以显著提高受精后的囊胚发育率（P〈0．05），可以用于牛体外受精胚胎的体外培养。
위연구표피생장인자（EGF）화이도소（Insulin）대우란모세포체외발육적영향이급체세포공배양대체외수정배태체외발육적영향，장획취적우란포란모세포재함유30μg，LEGF혹50mg／L Insulin혹30μg／LEGF＋50mg／L Insulin적성숙배양액중진행성숙배양，비교불동생장인자대우란모세포체외성숙화체외수정적영향；장수정란용과립세포단층배양계통혹우수란관상피세포단층배양계통진행체외배양，비교체세포공배양계통대체외수정배태체외발육적영향。결과표명，성숙배양액중첨가30μg／LEGF혹자30μg／LEGF＋50mg／L Insulin가이현저제고란모세포적성숙솔화수정후적란렬솔（P〈0．05）；수란관상피세포공배양계통가이현저제고수정후적낭배발육솔（P〈0．05），가이용우우체외수정배태적체외배양。
In this study, the effects of EGF and insulin, and different somatic cell co-culture systems on bovine in vitro fertilization （IVF） were investigated. The bovine oocytes were in vitro cultured with 30 μg/L EGF or 50 mg/L insulin or 30 μg/L EGF＋50 mg/L insulin, and the fertilized oocytes were cultured with grarmlosa cells or bovine oviduct epidermic ceils. The results show that the matured rate and cleavage rate significantly increased after added 30 μg/L EGF or 30μg/L EGF＋50 mg/L insulin to in vitro maturation medium, and the blastocyst rate significantly increased when the fertilized oocytes were cultured with bovine oviduct epidermic cells （P〈0.05） .