CAJ | 학술논문

为探索适宜枇杷的SSR反应体系，利用正交设计对Mg2＋、dNTPs、引物、Taq酶和模板DNA等5种因素4个水平进行筛选和优化。结果表明：20uL反应体系中，Mg2＋、dNTPs和引物的最适浓度分别为1．5mmol．L-1、0．15mmol·L-1、0．2umol·L-1，Taq酶最适用量为1U，模板DNA最适用量为20ng；引物的最佳退火温度为51．0～60．0℃。利用该反应体系对17份枇杷种质进行扩增，电泳结果显示，不同品种间DNA谱带多态性丰富，证实该体系稳定可靠。
위탐색괄의비파적SSR반응체계，이용정교설계대Mg2＋、dNTPs、인물、Taq매화모판DNA등5충인소4개수평진행사선화우화。결과표명：20uL반응체계중，Mg2＋、dNTPs화인물적최괄농도분별위1．5mmol．L-1、0．15mmol·L-1、0．2umol·L-1，Taq매최괄용량위1U，모판DNA최괄용량위20ng；인물적최가퇴화온도위51．0～60．0℃。이용해반응체계대17빈비파충질진행확증，전영결과현시，불동품충간DNA보대다태성봉부，증실해체계은정가고。
In this study, SSR condition on loquat was optimizatied. Orthogonal design was applied to optimize SSR -PCR amplification system of loquat in five factors such as Mg2＋ , dNTPs, primer , Taq polymerase and template DNA. The result showed that 1.5 mmol/L Mg2＋, 0. 15mmol/L dNTPs, 0. 2umol/L primer, 1uTaq polymerase, 20 ng template DNA and optimal annealing temperature of primers of 51.0 -60. 0℃ in 20uL SSR reaction system were the best. Trough above PCR system, SSR fragments of 17 loquat cuhivars were obtained and detected. Polymorphism between different cuhivars was abundantly detected, which showed this system was suitable and stable.