CAJ | 학술논문

背景:嗅鞘细胞移植治疗脊髓损伤在众多疗法中效果较佳,成为最有前景的治疗方法之一.目前移植方法为局部移植,存在操作复杂、创伤大、重复移植治疗困难等缺点.寻找一种简单易行且疗效好的移植方法成为各国学者研究的热点.目的:分析嗅鞘细胞静脉移植治疗脊髓损伤的可行性和疗效.方法:制备Wistar 大鼠脊髓半切模型,随机分4 组:嗅鞘细胞髓内局部移植组、嗅鞘细胞静脉移植组、D/F12 静脉移植组和空白对照组.定期行CBS 功能评分及组织学检查,评价脊髓修复情况.结果与结论:嗅鞘细胞髓内局部移植组、嗅鞘细胞静脉移植组的功能恢复和组织学改变优于D/F12 静脉移植组和空白对照组,嗅鞘细胞髓内局部移植组、嗅鞘细胞静脉移植组间无显著差别.说明嗅鞘细胞静脉移植可向脊髓损伤部位迁移并修复脊髓损伤,其疗效与嗅鞘细胞髓内局部移植相当.
배경:후초세포이식치료척수손상재음다요법중효과교가,성위최유전경적치료방법지일.목전이식방법위국부이식,존재조작복잡、창상대、중복이식치료곤난등결점.심조일충간단역행차료효호적이식방법성위각국학자연구적열점.목적:분석후초세포정맥이식치료척수손상적가행성화료효.방법:제비Wistar 대서척수반절모형,수궤분4 조:후초세포수내국부이식조、후초세포정맥이식조、D/F12 정맥이식조화공백대조조.정기행CBS 공능평분급조직학검사,평개척수수복정황.결과여결론:후초세포수내국부이식조、후초세포정맥이식조적공능회복화조직학개변우우D/F12 정맥이식조화공백대조조,후초세포수내국부이식조、후초세포정맥이식조간무현저차별.설명후초세포정맥이식가향척수손상부위천이병수복척수손상,기료효여후초세포수내국부이식상당.
BACKGROUND: Olfactory ensheathing cell transplantation is a better theray for spinal cord injury, and it becomes one of the most promising treatment methods. Local transplantation is applied currently, with the disadvantages of complex operation, large trauma, repeated transplantation. Looking for a simple and effective way for cell transplantation becomes a hotspot for scholars from various countries.OBJECTIVE: To investigate the effect and possibility of transplantation of olfactory ensheathing cells for treatment of spinal cord injury.METHODS: Wistar rats with T 10 spinal cord hemisecti on were divided into 4 groups: intramedullary local transplantation group (A),vein transplantation group (B), D/F12 transplantation group (C) and control group (D). The functional recovery of rats with spinal cord injury was observed with combined behavioral score at different phases. The tissue sections of each group were made at 5 and 10 weeks postoperatively to observe the axon regeneration and the survival of olfactory ensheathing cells.RESULTS AND CONCLUSION: The experiment showed that the rats transplanted with OECs at injured site and through the vein had more improvement in functional recovery and histological changes than the other two groups. The effect between A group and B group had not significant difference. The method of treating spinal cord injury by transplanting OECs via the vein not only simplifies the operation and avoids many complications but also has good curative effect similar to local transplantation.