中西医结合学报
中西醫結閤學報
중서의결합학보
JOURNAL OF CHINESE INTEGRATIVE MDEICINE
2011年
5期
565-569
,共5页
王琛%陆金根%银皓强%曹永清
王琛%陸金根%銀皓彊%曹永清
왕침%륙금근%은호강%조영청
皮下瘘%拖线疗法%中医%模型,动物
皮下瘺%拖線療法%中醫%模型,動物
피하루%타선요법%중의%모형,동물
subcutaneous fistula%thread-dragging therapy%traditional Chinese medicine%models,animal
目的:建立皮下瘘感染大鼠模型,并采用中医拖线疗法进行治疗.方法:24只雄性大鼠颈背部皮下植入弹簧纱条,并注入金黄色葡萄球菌和大肠杆菌混合菌液.45 d后在麻醉下取出弹簧纱条,经X线、超声、探针及病理检测验证皮下瘘感染造模成功.将模型大鼠随机分成两组,治疗组采用拖线疗法治疗,对照组采用瘘管切开术治疗,并对两组大鼠的创面愈合时间和大鼠体质量变化进行比较.结果:造模后45 d影像学证实有瘘管管道和外口存在,组织病理学检查显示有管腔及周围肉芽组织填充,与人类瘘管结构相似.治疗组创面平均愈合时间明显短于对照组(P＜0.01),术后7和14 d时治疗组大鼠的体质量较对照组大鼠偏重(P＜0.05).结论:用金黄色葡萄球菌和大肠杆菌混合菌感染的方法制作皮下瘘大鼠动物模型是简单可重复的.传统中医拖线疗法治疗皮下瘘大鼠损伤较小且安全.
目的:建立皮下瘺感染大鼠模型,併採用中醫拖線療法進行治療.方法:24隻雄性大鼠頸揹部皮下植入彈簧紗條,併註入金黃色葡萄毬菌和大腸桿菌混閤菌液.45 d後在痳醉下取齣彈簧紗條,經X線、超聲、探針及病理檢測驗證皮下瘺感染造模成功.將模型大鼠隨機分成兩組,治療組採用拖線療法治療,對照組採用瘺管切開術治療,併對兩組大鼠的創麵愈閤時間和大鼠體質量變化進行比較.結果:造模後45 d影像學證實有瘺管管道和外口存在,組織病理學檢查顯示有管腔及週圍肉芽組織填充,與人類瘺管結構相似.治療組創麵平均愈閤時間明顯短于對照組(P＜0.01),術後7和14 d時治療組大鼠的體質量較對照組大鼠偏重(P＜0.05).結論:用金黃色葡萄毬菌和大腸桿菌混閤菌感染的方法製作皮下瘺大鼠動物模型是簡單可重複的.傳統中醫拖線療法治療皮下瘺大鼠損傷較小且安全.
목적:건립피하루감염대서모형,병채용중의타선요법진행치료.방법:24지웅성대서경배부피하식입탄황사조,병주입금황색포도구균화대장간균혼합균액.45 d후재마취하취출탄황사조,경X선、초성、탐침급병리검측험증피하루감염조모성공.장모형대서수궤분성량조,치료조채용타선요법치료,대조조채용루관절개술치료,병대량조대서적창면유합시간화대서체질량변화진행비교.결과:조모후45 d영상학증실유루관관도화외구존재,조직병이학검사현시유관강급주위육아조직전충,여인류루관결구상사.치료조창면평균유합시간명현단우대조조(P＜0.01),술후7화14 d시치료조대서적체질량교대조조대서편중(P＜0.05).결론:용금황색포도구균화대장간균혼합균감염적방법제작피하루대서동물모형시간단가중복적.전통중의타선요법치료피하루대서손상교소차안전.
Objective: This study was to develop an experimental rat model of subcutaneous fistula induced by bacteria infection and treat it with thread-dragging therapy. Methods: A spring-gauze was surgically implanted into 24 male Sprague-Dawley rats' dorsal muscles, respectively, then mixed Staphylococcus aureus and Escherichia coli were injected into the spring-gauze. After 45 d, all the spring-gauzes were removed from the rats under anesthesia. X-ray and ultrasound were performed to assess the fistula tract. Each rat was examined by a fistula probe and four rats were randomly selected for histological assessment. The residual rats were randomly divided into two groups. Group A was treated with threaddragging therapy, and group B was treated with fistulotomy. The healing time and body weight of the two groups were compared. Results: On the 45th day of the spring-gauze implantation, imaging assessment proved a tract with two openings in all rats and histological results proved that lumen and surrounding granulation tissue with epithelium were similar to the features of fistula in human beings. The mean healing time of rats in group A was shorter than that of the rats in group B (P＜0. 01). The body weight of rats in group A was heavier than that of the rats in group B on the 7th and 14th day after surgery, respectively (P＜0.05).Conclusion: A rat model of subcutaneous fistula induced by mixed Staphylococcus aureus and Escherichia coli infection was successfully established. Traditional Chinese medicine threaddragging therapy is less invasive and safer than fistulotomy.
