CAJ | 학술논문

建立了一种利用双波长分光光度法测定烟酸酶法转化液中烟酸和6-羟基烟酸浓度的方法，选择的波长为266．5、300nm。对烟酸生物转化产物的混合物进行分析，烟酸测定结果的标准标准偏差不大于1．30％（n=6），6-羟基烟酸测定结果的相对标准标准偏差不大于1．54％（n=6）。烟酸和6-羟基烟酸的加标回收率为95．1％～105．1％．检出限分别为0．9mg／L和0．26mg／L。该法与常规的HPLC方法相比，快速、准确、适于大量样品的测定。
건립료일충이용쌍파장분광광도법측정연산매법전화액중연산화6-간기연산농도적방법，선택적파장위266．5、300nm。대연산생물전화산물적혼합물진행분석，연산측정결과적표준표준편차불대우1．30％（n=6），6-간기연산측정결과적상대표준표준편차불대우1．54％（n=6）。연산화6-간기연산적가표회수솔위95．1％～105．1％．검출한분별위0．9mg／L화0．26mg／L。해법여상규적HPLC방법상비，쾌속、준학、괄우대량양품적측정。
A method for determination of nicotinic acid and 6-hydroxynicotinic acid in enzymatic transformed mixture by double - wavelength UV spectrophotometry was developed, and the wavelength of 266.5 nm and 300 nm were selected. The relative standard deviation for nicotinic acid was less than 1.3% and 6-hydroxynicotinic acid was less than 1.54%. The recovery of nicotinic acid and 6-hydroxynicotinic acid was 95.1% - 105. I%. The detection limits for nicotinc and 6-hydroxynicotinic were 0.9 mg/L and 0.26 mg/L, respectively. The dual wavelength spectrophotometry is rapid, accurate and superior to conventional HPLC method, especially in bulk samples determination.