CAJ | 학술논문

背景:研究发现在炎症因子的刺激下内皮细胞和活化的T细胞表面表达CD146显著增加,故推测CD146可能参与了组织的炎症反应.目的:观察强直性脊柱炎患者外周血可溶性CD146的表达水平和临床意义.方法:选择上海交通大学附属第六人民医院住院的强直性脊柱炎患者62例,分为两组.活动期组46例,非活动组16例.同时选择同期医院健康职工和大学生20名为对照组.在强直性脊柱炎患者初诊时评估BASDAI、BASFI、强直性脊柱炎患者总体评分(PGA)、夜间痛、总体背痛评分、晨僵时间并测定红细胞沉降率、C-反应蛋白水平.应用ELISA法检测62例强直性脊柱炎患者与20例正常人外周血可溶性CD146的表达水平、魏氏法测定血沉及免疫比浊法测定C-反应蛋白.对强直性脊柱炎患者可溶性CD146与各项检测指标相关分析.结果与结论:强直性脊柱炎患者的外周血清可溶性CD146的表达水平较正常对照组显著增高(P＜0.05):活动期强直性脊柱炎患者可溶性CD146的表达水平较非活动期和正常对照组显著增高(P＜0.05);强直性脊柱炎患者血清可溶性CD146的表达水平与BASDAI呈正相关(P＜0.05);累及外周关节型者血清可溶性CD146较单独中轴型强直性脊柱炎患者和正常对照组显著增高(P＜0.05).结果表明,外周可溶性CD146表达水平的增加与强直性脊柱炎患者疾病的活动性有关,其可能参与了强直性脊柱炎的发病过程.
배경:연구발현재염증인자적자격하내피세포화활화적T세포표면표체CD146현저증가,고추측CD146가능삼여료조직적염증반응.목적:관찰강직성척주염환자외주혈가용성CD146적표체수평화림상의의.방법:선택상해교통대학부속제륙인민의원주원적강직성척주염환자62례,분위량조.활동기조46례,비활동조16례.동시선택동기의원건강직공화대학생20명위대조조.재강직성척주염환자초진시평고BASDAI、BASFI、강직성척주염환자총체평분(PGA)、야간통、총체배통평분、신강시간병측정홍세포침강솔、C-반응단백수평.응용ELISA법검측62례강직성척주염환자여20례정상인외주혈가용성CD146적표체수평、위씨법측정혈침급면역비탁법측정C-반응단백.대강직성척주염환자가용성CD146여각항검측지표상관분석.결과여결론:강직성척주염환자적외주혈청가용성CD146적표체수평교정상대조조현저증고(P＜0.05):활동기강직성척주염환자가용성CD146적표체수평교비활동기화정상대조조현저증고(P＜0.05);강직성척주염환자혈청가용성CD146적표체수평여BASDAI정정상관(P＜0.05);루급외주관절형자혈청가용성CD146교단독중축형강직성척주염환자화정상대조조현저증고(P＜0.05).결과표명,외주가용성CD146표체수평적증가여강직성척주염환자질병적활동성유관,기가능삼여료강직성척주염적발병과정.
BACKGROUND: Research has been reported that serum soluble CD146 (sCD146) expression was improved on the surface of endothelial cells and activated T cells by the stimulation of inflammatory factor. Therefore, it predicts that CD146 may participate in inflammatory reaction of tissue.OBJECTIVE: To investigate the expression and clinical significance of serum sCD146 in peripheral blood from patients with ankylosing spondylitis.METHODS: A total of 62 patients with ankylosing spondylitis were selected from the Sixth People's Hospital AffiUated to Shanghai Jiao Tong University. All patients were divided into two groups: active group (n=46) and inactive group (n=16); while, 20 healthy subjects were selected as the control group. Indicators including Bath Ankylosing SpondyUtis Disease ActivityIndex (BASDAI),Bath Ankylosing Spondylitis Functional Index (BASFI), patient's global assessment (PGA), night pain, visual analogue scale (VAS),morning stiffness time, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) were measured in all patients. The serum concentration of sCD146 from 62 patients with ankylosing spondlitis and 20 healthy controls was measured by enzyme-linked immunosorbent assay. Westergren method was used to measure ESR and immunoturbidimetry for CRP. Clinical data of the patients were collected as well.RESULTS AND CONCLUSION: sCD146 levels of patients with ankylosing spondlitis were significantly higher than normal control group (P ＜ 0.05). The sCD146 expression in the active group was significantly higher than inactive and normal control groups (P ＜0.05). Positive correlations were observed between sCD146 and BASDAI index of patients with ankylosing spondlitis (P ＜ 0.05).The sCD146 levels of ankylosing spondUtis patients with peripheral joint involvement were significantly higher than the patients with axial involvement alone or the normal controls (P ＜ 0.05).The expression level of sCD146 in peripheral blood was positively correlated with disease activities of patients with ankylosing spondlitis. It may play important roles in the pathogenesis in ankylosing spondlitis.