CAJ | 학술논문

本研究利用26对SRAP引物组合对46份甘蓝材料的遗传多样性和亲缘关系进行了分析,以期为甘蓝亲本选配提供参考.结果表明这26对引物共扩增出稳定清晰的条带439条,其中多态性条带为227条,多态性位点比率为51.7%.根据SRAP扩增结果,应用NTSYSpc2.1数据分析软件计算各品种间的遗传相似系数,结果表明这些材料间的遗传相似系数的变化范围是0.41～0.99.在相似性系数为0.568的水平上,可将46份甘蓝品种分为4大类,除第4类是形态特征明显有别于其它材料的抱子甘蓝外,其余3类结球甘蓝的主要聚类依据是其开花时间依次间隔一个星期左右.
본연구이용26대SRAP인물조합대46빈감람재료적유전다양성화친연관계진행료분석,이기위감람친본선배제공삼고.결과표명저26대인물공확증출은정청석적조대439조,기중다태성조대위227조,다태성위점비솔위51.7%.근거SRAP확증결과,응용NTSYSpc2.1수거분석연건계산각품충간적유전상사계수,결과표명저사재료간적유전상사계수적변화범위시0.41～0.99.재상사성계수위0.568적수평상,가장46빈감람품충분위4대류,제제4류시형태특정명현유별우기타재료적포자감람외,기여3류결구감람적주요취류의거시기개화시간의차간격일개성기좌우.
In this paper,we analyzed the genetic diversity and relationship among 46 cabbage germplasms by employing 26 SRAP primer combinations in order to provide reference for choice of parents for crossing in cabbage.The results showed that 439 amplified bands were obtained,among which 227 were polymorphic bands accounted for 51.7%polymorphism.The genetic similarity coefficients among these germplasm ranged from 0.41 to 0.99 by software NTSYSpc2.1 based on SRAP results.The 46 cabbage germplasm were divided into four groups on coefficient threshold of 0.568,among which three groups were clustered mainly according to flowering interval of about one week,while the fourth group consisted of brussel sprout and showed obvious differences from other germplasm in morphological characters.